On-Chip Assay for Home-Sampling, Mail-Based Shipping and Centralized Laboratory Readout

• On-chip immunoassays have great potential to accelerate point-of-care (POC) testing. However, most attempts require either impractical readout equipment or are outperformed by the sensitivity, reliability and affordability offered by centralized laboratories. Here, we present a capillary-driven microfluidic device that unites the best of two worlds: Bead-based analyte isolation at the POC, where analyte drying enables simple mail-based shipping, combined with the powerful capabilities of centralized laboratories. Using C-reactive protein (CRP) as model biomarker we demonstrate on-chip assay performance similar to conventional assays, with a LOD of 10 pg/ml and average CV of 9.7 % (48 microfluidic devices).

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

TEKNIK OCH TEKNOLOGIER  -- Medicinteknik -- Medicinsk laboratorie- och mätteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Medical Engineering -- Medical Laboratory and Measurements Technologies (hsv//eng)

Keyword

Beads

CRP

Immunoassay

Point-Of-Care (POC)

Sample preparation

Publication and Content Type

ref (subject category)

kon (subject category)