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Multiple enzymatic ...
Multiple enzymatic approaches to hydrolysis of fungal β-glucans by the soil bacterium Chitinophaga pinensis
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- Lu, Zijia (author)
- KTH,Glykovetenskap
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- Rämgård, Carl (author)
- KTH,Glykovetenskap
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- Ergenlioğlu, İrem (author)
- KTH,Glykovetenskap
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- Sandin, Lova (author)
- KTH,Glykovetenskap
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- Hammar, Hugo (author)
- KTH,Glykovetenskap
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- Andersson, Helena (author)
- KTH,Glykovetenskap
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- King, Katharine (author)
- KTH,Glykovetenskap
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- Inman, Annie R. (author)
- KTH,Glykovetenskap
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- Hao, Mengshu (author)
- KTH,Glykovetenskap
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- Bulone, Vincent (author)
- KTH,Glykovetenskap,College of Medicine & Public Health, Flinders University, Adelaide, SA, Australia
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- McKee, Lauren S., 1985- (author)
- KTH,Glykovetenskap,Wallenberg Wood Science Center
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(creator_code:org_t)
- 2023-01-24
- 2023
- English.
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In: The FEBS Journal. - : Wiley. - 1742-464X .- 1742-4658. ; 290:11, s. 2909-2922
- Related links:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The genome of the soil Bacteroidota Chitinophaga pinensis encodes a large number of glycoside hydrolases (GHs) with noteworthy features and potentially novel functions. Several are predicted to be active on polysaccharide components of fungal and oomycete cell walls, such as chitin, β-1,3-glucan and β-1,6-glucan. While several fungal β-1,6-glucanase enzymes are known, relatively few bacterial examples have been characterised to date. We have previously demonstrated that C. pinensis shows strong growth using β-1,6-glucan as the sole carbon source, with the efficient release of oligosaccharides from the polymer. We here characterise the capacity of the C. pinensis secretome to hydrolyse the β-1,6-glucan pustulan and describe three distinct enzymes encoded by its genome, all of which show different levels of β-1,6-glucanase activity and which are classified into different GH families. Our data show that C. pinensis has multiple tools to deconstruct pustulan, allowing the species' broad utility of this substrate, with potential implications for bacterial biocontrol of pathogens via cell wall disruption. Oligosaccharides derived from fungal β-1,6-glucans are valuable in biomedical research and drug synthesis, and these enzymes could be useful tools for releasing such molecules from microbial biomass, an underexploited source of complex carbohydrates.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Annan kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Other Chemistry Topics (hsv//eng)
Keyword
- carbohydrate-binding module
- glycoside hydrolase
- pustulan
- β-1
- 3-glucanase
- β-1
- 6-glucanase
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Lu, Zijia
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Rämgård, Carl
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Ergenlioğlu, İre ...
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Sandin, Lova
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Hammar, Hugo
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Andersson, Helen ...
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show more...
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King, Katharine
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Inman, Annie R.
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Hao, Mengshu
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Bulone, Vincent
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McKee, Lauren S. ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Other Chemistry ...
- Articles in the publication
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The FEBS Journal
- By the university
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Royal Institute of Technology