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Probabilistic cell ...
Probabilistic cell seeding and non-autofluorescent 3D-printed structures as scalable approach for multi-level co-culture modeling
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- Buchmann, Sebastian (author)
- Karolinska Institutet,KTH,Nanobioteknologi
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- Enrico, Alessandro (author)
- KTH,Mikro- och nanosystemteknik
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- Holzreuter, Muriel Alexandra (author)
- KTH,Mikro- och nanosystemteknik
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- Reid, Michael S. (author)
- KTH,Fiberteknologi
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- Zeglio, Erica (author)
- KTH,Nanobioteknologi
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- Niklaus, Frank, 1971- (author)
- KTH,Mikro- och nanosystemteknik
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- Stemme, Göran, 1958- (author)
- KTH,Mikro- och nanosystemteknik
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- Herland, Anna (author)
- Karolinska Institutet,KTH,Nanobioteknologi
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(creator_code:org_t)
- Elsevier BV, 2023
- 2023
- English.
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In: Materials Today Bio. - : Elsevier BV. - 2590-0064. ; 21, s. 100706-100706
- Related links:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- To model complex biological tissue in vitro, a specific layout for the position and numbers of each cell type isnecessary. Establishing such a layout requires manual cell placement in three dimensions (3D) with micrometricprecision, which is complicated and time-consuming. Moreover, 3D printed materials used in compartmentalizedmicrofluidic models are opaque or autofluorescent, hindering parallel optical readout and forcing serial charac-terization methods, such as patch-clamp probing. To address these limitations, we introduce a multi-level co-culture model realized using a parallel cell seeding strategy of human neurons and astrocytes on 3D structuresprinted with a commercially available non-autofluorescent resin at micrometer resolution. Using a two-stepstrategy based on probabilistic cell seeding, we demonstrate a human neuronal monoculture that forms net-works on the 3D printed structure and can establish cell-projection contacts with an astrocytic-neuronal co-cultureseeded on the glass substrate. The transparent and non-autofluorescent printed platform allows fluorescence-based immunocytochemistry and calcium imaging. This approach provides facile multi-level compartmentaliza-tion of different cell types and routes for pre-designed cell projection contacts, instrumental in studying complextissue, such as the human brain.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biomaterial (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bio Materials (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- Two-photon polymerization Neurons Astrocytes Calcium imaging Co-culture models IP-Visio
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Buchmann, Sebast ...
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Enrico, Alessand ...
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Holzreuter, Muri ...
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Reid, Michael S.
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Zeglio, Erica
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Niklaus, Frank, ...
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show more...
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Stemme, Göran, 1 ...
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Herland, Anna
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- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Nano technology
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
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and Bio Materials
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
- Articles in the publication
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Materials Today ...
- By the university
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Royal Institute of Technology
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Karolinska Institutet