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Transcriptomic prof...
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Röhl, SamuelDepartment of Molecular Medicine and Surgery, Karolinska Institutet, Solna, Sweden
(author)
Transcriptomic profiling of experimental arterial injury reveals new mechanisms and temporal dynamics in vascular healing response
- Article/chapterEnglish2020
Publisher, publication year, extent ...
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Elsevier BV,2020
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-332038
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-332038URI
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https://doi.org/10.1016/j.jvssci.2020.01.001DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:234617037URI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:148679870URI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20230718
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Objective: Endovascular interventions cause arterial injury and induce a healing response to restore vessel wall homeostasis. Complications of defective or excessive healing are common and result in increased morbidity and repeated interventions. Experimental models of intimal hyperplasia are vital for understanding the vascular healing mechanisms and resolving the clinical problems of restenosis, vein graft stenosis, and dialysis access failure. Our aim was to systematically investigate the transcriptional, histologic, and systemic reaction to vascular injury during a prolonged time. Methods: Balloon injury of the left common carotid artery was performed in male rats. Animals (n = 69) were euthanized before or after injury, either directly or after 2 hours, 20 hours, 2 days, 5 days, 2 weeks, 6 weeks, and 12 weeks. Both injured and contralateral arteries were subjected to microarray profiling, followed by bioinformatic exploration, histologic characterization of the biopsy specimens, and plasma lipid analyses. Results: Immune activation and coagulation were key mechanisms in the early response, followed by cytokine release, tissue remodeling, and smooth muscle cell modulation several days after injury, with reacquisition of contractile features in later phases. Novel pathways related to clonal expansion, inflammatory transformation, and chondro-osteogenic differentiation were identified and immunolocalized to neointimal smooth muscle cells. Analysis of uninjured arteries revealed a systemic component of the reaction after local injury, underlined by altered endothelial signaling, changes in overall tissue bioenergy metabolism, and plasma high-density lipoprotein levels. Conclusions: We demonstrate that vascular injury induces dynamic transcriptional landscape and metabolic changes identifiable as early, intermediate, and late response phases, reaching homeostasis after several weeks. This study provides a temporal “roadmap” of vascular healing as a publicly available resource for the research community.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Rykaczewska, UrszulaKarolinska Institutet
(author)
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Seime, TillDepartment of Molecular Medicine and Surgery, Karolinska Institutet, Solna, Sweden
(author)
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Suur, Bianca E.Karolinska Institutet
(author)
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Diez, Maria GonzalezDepartment of Medicine, Karolinska Institutet, Solna, Sweden
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Gådin, Jesper R.Department of Medicine, Karolinska Institutet, Solna, Sweden
(author)
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Gainullina, AnastasiiaITMO University, St. Petersburg, Russia, Department of Computer Technologies, ITMO University
(author)
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Sergushichev, Alexey A.ITMO University, St. Petersburg, Russia, Department of Computer Technologies, ITMO University
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Wirka, RobertDepartment of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, Calif
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Lengquist, MarietteKarolinska Institutet
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Kronqvist, MalinKarolinska Institutet
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Bergman, OttoKarolinska Institutet
(author)
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Odeberg, Jacob,Professor,1963-KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap,Science for Life Laboratory, Department of Protein Science, School of Chemistry, Biotechnology and Health, Royal Institute of Technology, Sweden, and Coagulation Unit, Department of Haematology, Karolinska University Hospital, Stockholm, Sweden(Swepub:kth)u1e4yljo
(author)
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Lindeman, Jan H.N.Department of Haematology, Coagulation Unit, Karolinska University Hospital
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Quertermous, ThomasDepartment of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, Calif
(author)
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Hamsten, AndersKarolinska Institutet
(author)
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Eriksson, PerKarolinska Institutet
(author)
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Hedin, UlfKarolinska Institutet
(author)
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Razuvaev, AntonKarolinska Institutet
(author)
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Matic, Ljubica PerisicKarolinska Institutet
(author)
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Karolinska InstitutetDepartment of Molecular Medicine and Surgery, Karolinska Institutet, Solna, Sweden
(creator_code:org_t)
Related titles
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In:JVS-Vascular Science: Elsevier BV315, s. E14-E142666-3503
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In:ATHEROSCLEROSIS: Elsevier BV315, s. E14-E140021-9150
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Röhl, Samuel
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Rykaczewska, Urs ...
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Seime, Till
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Suur, Bianca E.
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Diez, Maria Gonz ...
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Gådin, Jesper R.
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Gainullina, Anas ...
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Sergushichev, Al ...
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Wirka, Robert
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Lengquist, Marie ...
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Kronqvist, Malin
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Bergman, Otto
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Odeberg, Jacob, ...
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Lindeman, Jan H. ...
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Quertermous, Tho ...
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Hamsten, Anders
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Eriksson, Per
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Hedin, Ulf
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Razuvaev, Anton
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Matic, Ljubica P ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Surgery
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JVS-Vascular Sci ...
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ATHEROSCLEROSIS
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Royal Institute of Technology
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Karolinska Institutet