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In vitro models to study natural killer cell dynamics in the tumor microenvironment
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- Carannante, Valentina (author)
- KTH,Biofysik
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- Wiklund, Martin (author)
- KTH,Science for Life Laboratory, SciLifeLab,Biofysik
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- Önfelt, Björn (author)
- KTH,Science for Life Laboratory, SciLifeLab,Biofysik,Karolinska Inst, Ctr Infect Med, Dept Med Huddinge, Sci Life Lab, Stockholm, Sweden.
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(creator_code:org_t)
- Frontiers Media SA, 2023
- 2023
- English.
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In: Frontiers in Immunology. - : Frontiers Media SA. - 1664-3224. ; 14
- Related links:
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https://doi.org/10.3...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Immunotherapy is revolutionizing cancer therapy. The rapid development of new immunotherapeutic strategies to treat solid tumors is posing new challenges for preclinical research, demanding novel in vitro methods to test treatments. Such methods should meet specific requirements, such as enabling the evaluation of immune cell responses like cytotoxicity or cytokine release, and infiltration into the tumor microenvironment using cancer models representative of the original disease. They should allow high-throughput and high-content analysis, to evaluate the efficacy of treatments and understand immune-evasion processes to facilitate development of new therapeutic targets. Ideally, they should be suitable for personalized immunotherapy testing, providing information for patient stratification. Consequently, the application of in vitro 3-dimensional (3D) cell culture models, such as tumor spheroids and organoids, is rapidly expanding in the immunotherapeutic field, coupled with the development of novel imaging-based techniques and -omic analysis. In this paper, we review the recent advances in the development of in vitro 3D platforms applied to natural killer (NK) cell-based cancer immunotherapy studies, highlighting the benefits and limitations of the current methods, and discuss new concepts and future directions of the field.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- NK cells
- tumor microenvironment
- tumor spheroids
- tumor organoids
- microscopy
- flow cytometry
- tissue sectioning
- live cell imaging
Publication and Content Type
- ref (subject category)
- for (subject category)
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