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Root and Leaf Anato...
Root and Leaf Anatomy, Ion Accumulation, and Transcriptome Pattern under Salt Stress Conditions in Contrasting Genotypes of Sorghum bicolor
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- Karumanchi, Appa Rao (author)
- Acharya Nagarjuna Univ, Dept Biotechnol, Guntur 522209, India.
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- Sivan, Pramod, 1984- (author)
- KTH,Glykovetenskap
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- Kummari, Divya (author)
- Int Crops Res Inst Semi Arid Trop, Hyderabad 502324, India.
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- Rajasheker, G. (author)
- Osmania Univ, Dept Genet, Hyderabad 500007, India.
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- Kumar, S. Anil (author)
- Vignans Fdn Sci Technol & Res Deemed Univ, Dept Biotechnol, Guntur 522213, India.
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- Reddy, Palakolanu Sudhakar (author)
- Int Crops Res Inst Semi Arid Trop, Hyderabad 502324, India.
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- Suravajhala, Prashanth (author)
- Amrita Vishwa Vidyapeetham, Amrita Sch Biotechnol, Kollam 690525, India.
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- Podha, Sudhakar (author)
- Acharya Nagarjuna Univ, Dept Biotechnol, Guntur 522209, India.
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- Kishor, P. B. Kavi (author)
- Osmania Univ, Dept Genet, Hyderabad 500007, India.
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Acharya Nagarjuna Univ, Dept Biotechnol, Guntur 522209, India Glykovetenskap (creator_code:org_t)
- MDPI AG, 2023
- 2023
- English.
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In: PLANTS. - : MDPI AG. - 2223-7747. ; 12:13
- Related links:
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https://doi.org/10.3...
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https://urn.kb.se/re...
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Abstract
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- Roots from salt-susceptible ICSR-56 (SS) sorghum plants display metaxylem elements with thin cell walls and large diameter. On the other hand, roots with thick, lignified cell walls in the hypodermis and endodermis were noticed in salt-tolerant CSV-15 (ST) sorghum plants. The secondary wall thickness and number of lignified cells in the hypodermis have increased with the treatment of sodium chloride stress to the plants (STN). Lignin distribution in the secondary cell wall of sclerenchymatous cells beneath the lower epidermis was higher in ST leaves compared to the SS genotype. Casparian thickenings with homogenous lignin distribution were observed in STN roots, but inhomogeneous distribution was evident in SS seedlings treated with sodium chloride (SSN). Higher accumulation of K+ and lower Na+ levels were noticed in ST compared to the SS genotype. To identify the differentially expressed genes among SS and ST genotypes, transcriptomic analysis was carried out. Both the genotypes were exposed to 200 mM sodium chloride stress for 24 h and used for analysis. We obtained 70 and 162 differentially expressed genes (DEGs) exclusive to SS and SSN and 112 and 26 DEGs exclusive to ST and STN, respectively. Kyoto Encyclopaedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analysis unlocked the changes in metabolic pathways in response to salt stress. qRT-PCR was performed to validate 20 DEGs in each SSN and STN sample, which confirms the transcriptomic results. These results surmise that anatomical changes and higher K+/Na+ ratios are essential for mitigating salt stress in sorghum apart from the genes that are differentially up- and downregulated in contrasting genotypes.
Subject headings
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
Keyword
- ion accumulation
- root and leaf anatomy
- sorghum
- salt stress
- transcriptome
Publication and Content Type
- ref (subject category)
- art (subject category)
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Karumanchi, Appa ...
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Sivan, Pramod, 1 ...
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Kummari, Divya
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Rajasheker, G.
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Kumar, S. Anil
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Reddy, Palakolan ...
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Suravajhala, Pra ...
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Podha, Sudhakar
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Kishor, P. B. Ka ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Botany
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PLANTS
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Royal Institute of Technology