SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:DiVA.org:kth-335096"
 

Search: onr:"swepub:oai:DiVA.org:kth-335096" > Ovarian ERβ cistrom...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003154nam a2200325 4500
001oai:DiVA.org:kth-335096
003SwePub
008230907| | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3350962 URI
040 a (SwePub)kth
041 a engb eng
042 9 SwePub
072 7a vet2 swepub-contenttype
072 7a ovr2 swepub-publicationtype
1001 a Birgersson, Madeleineu KTH,Proteinvetenskap4 aut0 (Swepub:kth)u1cwmzu4
2451 0a Ovarian ERβ cistrome and transcriptome reveal chromatin interaction with LRH-1
338 a print2 rdacarrier
500 a QC 20230908
520 a Background:  Estrogen receptor beta (ERβ, Esr2) plays a pivotal role in folliculogenesis and ovulation, yet its exact mechanism of action is mainly uncharacterized.Results: We here performed ChIP-sequencing of mouse ovaries followed by complementary RNA-sequencing of wild-type and ERβ knockout ovaries. By integrating the cistrome and transcriptome, we identify its direct target genes and enriched biological functions in the ovary. This demonstrates a strong impact on genes regulating organism development, cell migration, lipid metabolism, response to hypoxia, and response to estrogen. Cell-type deconvolution analysis of the bulk RNA-seq data revealed a decrease in luteal cells and an increased proportion of theca cells and a specific type of cumulus cells upon ERβ loss. Moreover, we identify a significant overlap with the gene regulatory network of liver receptor homolog 1 (LRH-1). ERβ and LRH-1 extensively bind to the same chromatin locations in granulosa cells and we corroborate simultaneous co-binding using ChIP re-ChIP, at the ERβ-repressed gene Greb1. At other shared sites (by ERβ-upregulated genes Cyp11a1 and Fkbp5), they do not bind simultaneously. Transactivation assay experimentation further show that ERβ and LRH-1 can inhibit their respective transcriptional activity at classical response elements.Conclusions: We characterize genome-wide ERβ chromatin binding and gene regulations which reveal extensive crosstalk between ERβ and LRH-1. We experimentally corroborate co-binding to target genes and impact on transactivation. Our data offer genome-wide mechanistic underpinnings of ovarian physiology and fertility.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Medicinsk bioteknologi0 (SwePub)304012 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Medical Biotechnology0 (SwePub)304012 hsv//eng
7001 a Indukuri, Rajithau KTH,Cellulär och klinisk proteomik4 aut0 (Swepub:kth)u1h2gbg8
7001 a Lindquist, Linnea4 aut
7001 a Stepanauskaite, Linau KTH,Cellulär och klinisk proteomik4 aut0 (Swepub:kth)u1xd7zej
7001 a Luo, Qing4 aut
7001 a Deng, Qiaolin4 aut
7001 a Archer, Amenau KTH,Cellulär och klinisk proteomik4 aut0 (Swepub:kth)u131bapq
7001 a Williams, Cecilia,c Professor,d 1969-u KTH,Cellulär och klinisk proteomik4 aut0 (Swepub:kth)u1ygqmuy
7102 a KTHb Proteinvetenskap4 org
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-335096

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view