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Spatial transcripto...
Spatial transcriptomics of B cell and T cell receptors reveals lymphocyte clonal dynamics
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- Engblom, Camilla (author)
- Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
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- Thrane, Kim (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Lin, Qirong (author)
- Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
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- Andersson, Alma (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Toosi, Hosein (author)
- KTH,Beräkningsvetenskap och beräkningsteknik (CST),Science for Life Laboratory, SciLifeLab
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- Chen, Xinsong (author)
- Karolinska Institutet
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- Steiner, Embla (author)
- Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
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- Lu, Chang (author)
- Heidelberg University, Faculty of Medicine and Heidelberg University Hospital, Institute for Computational Biomedicine, Heidelberg, Germany.
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- Mantovani, Giulia (author)
- Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
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- Hagemann-Jensen, Michael (author)
- Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
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- Saarenpää, Sami (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Jangard, Mattias (author)
- ENT Unit, Sophiahemmet University Research Laboratory and Sophiahemmet Hospital, Stockholm, Sweden.
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- Saez-Rodriguez, Julio (author)
- Heidelberg University, Faculty of Medicine and Heidelberg University Hospital, Institute for Computational Biomedicine, Heidelberg, Germany.
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- Michaëlsson, Jakob (author)
- Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden.
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- Hartman, Johan (author)
- Karolinska Institutet
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- Lagergren, Jens (author)
- KTH,Beräkningsvetenskap och beräkningsteknik (CST),Science for Life Laboratory, SciLifeLab
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- Mold, Jeff E. (author)
- Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
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- Lundeberg, Joakim (author)
- KTH,Science for Life Laboratory, SciLifeLab,Genteknologi
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- Frisén, Jonas (author)
- Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
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Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden Genteknologi (creator_code:org_t)
- American Association for the Advancement of Science (AAAS), 2023
- 2023
- English.
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In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 382:6675, s. 8486-
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- The spatial distribution of lymphocyte clones within tissues is critical to their development, selection, and expansion. We have developed spatial transcriptomics of variable, diversity, and joining (VDJ) sequences (Spatial VDJ), a method that maps B cell and T cell receptor sequences in human tissue sections. Spatial VDJ captures lymphocyte clones that match canonical B and T cell distributions and amplifies clonal sequences confirmed by orthogonal methods. We found spatial congruency between paired receptor chains, developed a computational framework to predict receptor pairs, and linked the expansion of distinct B cell clones to different tumor-associated gene expression programs. Spatial VDJ delineates B cell clonal diversity and lineage trajectories within their anatomical niche. Thus, Spatial VDJ captures lymphocyte spatial clonal architecture across tissues, providing a platform to harness clonal sequences for therapy.
Subject headings
- NATURVETENSKAP -- Biologi -- Utvecklingsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Developmental Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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Science
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- By the author/editor
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Engblom, Camilla
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Thrane, Kim
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Lin, Qirong
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Andersson, Alma
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Toosi, Hosein
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Chen, Xinsong
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show more...
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Steiner, Embla
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Lu, Chang
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Mantovani, Giuli ...
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Hagemann-Jensen, ...
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Saarenpää, Sam ...
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Jangard, Mattias
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Saez-Rodriguez, ...
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Michaëlsson, Jak ...
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Hartman, Johan
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Lagergren, Jens
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Mold, Jeff E.
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Lundeberg, Joaki ...
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Frisén, Jonas
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Developmental Bi ...
- Articles in the publication
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Science
- By the university
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Royal Institute of Technology
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Karolinska Institutet