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Rapid Screening of ...
Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
- Article/chapterEnglish2011
Publisher, publication year, extent ...
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2011-05-19
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Public Library of Science (PLoS),2011
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-34357
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-34357URI
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https://doi.org/10.1371/journal.pone.0019723DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:122606770URI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20110804
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Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Zheng, ZongliKarolinska Institutet
(author)
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Andersson, Anders F.KTH,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1hi776x
(author)
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Engstrand, LarsKarolinska Institutet
(author)
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Ye, WeiminKarolinska Institutet
(author)
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Karolinska InstitutetScience for Life Laboratory, SciLifeLab
(creator_code:org_t)
Related titles
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In:PLOS ONE: Public Library of Science (PLoS)6:5, s. e19723-1932-6203
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