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  • Huang, Jiaqi (author)

Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing

  • Article/chapterEnglish2011

Publisher, publication year, extent ...

  • 2011-05-19
  • Public Library of Science (PLoS),2011
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:kth-34357
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-34357URI
  • https://doi.org/10.1371/journal.pone.0019723DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:122606770URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • QC 20110804
  • Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Zheng, ZongliKarolinska Institutet (author)
  • Andersson, Anders F.KTH,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1hi776x (author)
  • Engstrand, LarsKarolinska Institutet (author)
  • Ye, WeiminKarolinska Institutet (author)
  • Karolinska InstitutetScience for Life Laboratory, SciLifeLab (creator_code:org_t)

Related titles

  • In:PLOS ONE: Public Library of Science (PLoS)6:5, s. e19723-1932-6203

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