Search: onr:"swepub:oai:DiVA.org:kth-344913" >
Widespread enzyme e...
Widespread enzyme expression variations underlie diverse metabolic capacities within cell types
-
- Gnann, Christian (author)
- KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab
-
- Cesnik, Anthony J. (author)
- KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab
-
Le, Trang (author)
-
show more...
-
Sariyar, Sanem (author)
-
Sigaeva, Alina (author)
-
Mahdessian, Diana (author)
-
Schutten, Rutger (author)
-
Raghavan, Preethi (author)
-
Leonetti, Manuel D (author)
-
Lindskog, Cecilia (author)
-
- Uhlén, Mathias (author)
- KTH,Systembiologi
-
Axelsson, Ulrika (author)
-
Lundberg, Emma (author)
-
show less...
-
(creator_code:org_t)
- English.
- Related links:
-
https://urn.kb.se/re...
Abstract
Subject headings
Close
- Metabolic enzymes perform life-sustaining functions in various compartments of the cell. Recent studies have shown some enzymes to exhibit varied expression or localization between genetically identical cells and that this heterogeneity impacts drug resistance, metastasis, differentiation, and immune cell activation. However, no systematic analysis of metabolic cellular heterogeneity has been performed. Here, we leverage imaging-based single-cell spatial proteomic data to reveal the extent of non-genetic partitioning of the metabolic proteome. Over half of all enzymes localize to multiple cellular compartments, hinting at moonlighting potential. In addition, nearly two fifths of metabolic enzymes exhibit cell-to-cell variable expression. We demonstrate that individual cells reproduce these highly heterogeneous cell populations using clonal expansion, establishing that cells recapitulate myriad metabolic phenotypes over just a few cell divisions. To identify multifunctional moonlighting enzymes, we mine protein-protein interaction datasets to find interacting proteins with distinct functional roles, and using a timeresolved transcriptomic dataset, we find that metabolic heterogeneity arises largely independently of cell cycle progression and is established mostly post-transcriptionally or posttranslationally. Taken together, our data suggest that the heterogeneity of metabolic enzymes establish diverse cellular phenotypes, which are reflected in tissues, and which may ultimately allow targeted studies of their roles in health and disease.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Publication and Content Type
- vet (subject category)
- ovr (subject category)
To the university's database
- By the author/editor
-
Gnann, Christian
-
Cesnik, Anthony ...
-
Le, Trang
-
Sariyar, Sanem
-
Sigaeva, Alina
-
Mahdessian, Dian ...
-
show more...
-
Schutten, Rutger
-
Raghavan, Preeth ...
-
Leonetti, Manuel ...
-
Lindskog, Cecili ...
-
Uhlén, Mathias
-
Axelsson, Ulrika
-
Lundberg, Emma
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
- By the university
-
Royal Institute of Technology