Cryopreservation of stem cell-derived pancreatic islets using twisted vitrification with a supporting FN-silk network

(creator_code:org_t)

Abstract Subject headings

Cryopreservation is crucial for making cell therapies more accessible by enabling long-term storage and facilitating transplantation logistics. However, preserving cells in 3D models implies significant challenges, including inhomogeneous penetration of cryoprotectants and non-uniform heat transfer during freezing and thawing. Vitrification is a novel cryopreservation approach that improves the survival of living cells by providing optimal cooling and rewarming rates to eliminate ice crystal-induced mechanical injury. Nevertheless, sterility issues can arise from direct contact with liquid nitrogen (LN2). The twisted vitrification method addresses this by separating the cells and LN2 into two different compartments, ensuring a sterile vitrification process.In this study, we optimized the cryopreservation of stem cell-derived pancreatic islets (SC-islets) by combining twisted vitrification with the supporting biomaterial FN-silk. We compared the viability of free SC-islets to those supported by a FN-silk network during cryopreservation using either conventional slow freezing or twisted vitrification. Our results showed a higher post-recovery for SC-islets incorporated in FN-silk using twisted vitrification, outperforming both conventional slow freezing and twisted vitrification of free SC-islets. Additionally, insulin and glucagon expression were maintained after vitrification within the FN-silk network.

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Biomaterials Sci ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Medical Biotechn ...

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