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Non-invasive evalua...
Non-invasive evaluation of alginate/poly-L-lysine/alginate microcapsules by magnetic resonance microscopy
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Constantinidis, Ioannis (author)
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Grant, Samuel C. (author)
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- Celper, Susanne (author)
- KTH
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- Gauffin-Holmberg, Isabel (author)
- KTH
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- Agering, Kristina (author)
- KTH
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Oca-Cossio, Jose A. (author)
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Bui, Jonathan D. (author)
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Flint, Jeremy (author)
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Hamaty, Christine (author)
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Simpson, Nicholas E. (author)
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Blackband, Stephen J. (author)
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- English.
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In: Biomaterials. - : Elsevier BV. - 0142-9612 .- 1878-5905. ; 28:15, s. 2438-2445
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- In this report, we present data to demonstrate the utility of H-1 MR microscopy to non-invasively examine alginate/poly-L-lysine/ alginate (APA) microcapsules. Specifically, high-resolution images were used to visualize and quantify the poly-L-lysine (PLL) layer, and monitor temporal changes in the alginate gel microstructure during a month long in vitro culture. The thickness of the alginate/PLL layer was quantified to be 40.6 +/- 6.2 mu m regardless of the alginate composition used to generate the beads or the time of alginate/PLL interaction (2, 6, or 20 min). However, there was a notable difference in the contrast of the PLL layer that depended upon the guluronic content of the alginate and the alginate/PLL interaction time. The T-2 relaxation time and the apparent diffusion coefficient (ADC) of the alginate matrix were measured periodically throughout the month long culture period. Alginate beads generated with a high guluronic content alginate demonstrated a temporal decrease in T-2 over the duration of the experiment, while ADC was unaffected. This decrease in T-2 is attributed to a reorganization of the alginate microstructure due to periodic media exchanges that mimicked a regular feeding regiment for cultured cells. In beads coated with a PLL layer, this temporal decrease in T-2 was less pronounced suggesting that the PLL layer helped maintain the integrity of the initial alginate microstructure. Conversely, alginate beads generated with a high mannuronic content alginate (with or without a PLL layer) did not display temporal changes in either T-2 or ADC. This observation suggests that the microstructure of high mannuronic content alginate beads is less susceptible to culture conditions.
Keyword
- MR imaging
- alginate encapsulation
- poly-L-lysine
- TECHNOLOGY
- TEKNIKVETENSKAP
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Constantinidis, ...
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Grant, Samuel C.
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Celper, Susanne
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Gauffin-Holmberg ...
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Agering, Kristin ...
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Oca-Cossio, Jose ...
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show more...
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Bui, Jonathan D.
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Flint, Jeremy
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Hamaty, Christin ...
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Simpson, Nichola ...
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Blackband, Steph ...
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show less...
- Articles in the publication
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Biomaterials
- By the university
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Royal Institute of Technology
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Karolinska Institutet