Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides

• BioRad's Rotofor (R) system has been frequently used for the purification of proteins and smaller pepticles such as bacteriocins. In this study, we report that some commercially available ampholytes used with the Rotofor (R) isoelectric focusing system possess antimicrobial activity, which may interfere with the purification of bacteriocins and bacteriocin-like substances.

Subject headings

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Keyword

ampholytes

rotofor

isoelectric focusing

bacteriocins

Biology

Biologi

Publication and Content Type

ref (subject category)

art (subject category)