onr:"swepub:oai:DiVA.org:kth-37621"
Search: onr:"swepub:oai:DiVA.org:kth-37621" > Biochemical consequ...
- 1 of 1
- Previous record
- Next record
- To hitlist
Biochemical consequences of alginate encapsulation : A NMR study of insulin-secreting cells
- Simpson, N. E. (author)
- Grant, S. C. (author)
-
- Gustavsson, Lenita (author)
- KTH
- show more...
- Blackband, S. J. (author)
- Constantinidis, I. (author)
- show less...
- (creator_code:org_t)
- Elsevier BV, 2006
- 2006
- English.
- In: Biomaterials. - : Elsevier BV. - 0142-9612 .- 1878-5905. ; 27:12, s. 2577-2586
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- In this study we explore the biochemical consequences of alginate encapsulation on beta TC3 cells. C-13 NMR spectroscopy and isotopomer analysis were used to investigate the effects of encapsulation on several enzymatic processes associated with the TCA cycle. Our data show statistically significant differences in various enzymatic fluxes related to the TCA cycle and insulin secretion between monolayer and algainate-encapsulated cultures. The principal cause for these effects was the process of trypsinization. Embedding the trypsinized cells in alginate beads did not have a compounded effect on the enzymatic fluxes of entrapped cells. However, an additional small but statistically significant decrease in insulin secretion was measured in encapsulated cells. Finally, differences in either enzymatic fluxes or Qlucose consumption as a function of bead diameter were not observed. However, differences in T-2, assessed by H-1 NMR microimaging, were observed as a function of bead diameter, suggesting that smaller beads became more organized with time in culture, while larger beads displayed a looser organization.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- alginate
- metabolism
- C-13 NMR
- isotopomer
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Simpson, N. E.
- Grant, S. C.
- Gustavsson, Leni ...
- Peltonen, Vilje- ...
- Blackband, S. J.
- Constantinidis, ...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- Articles in the publication
- Biomaterials
- By the university
- Royal Institute of Technology
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
