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Biosticker : patterned microfluidic stickers for rapid integration with microarrays
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- Carlborg, Carl Fredrik (author)
- KTH,Mikrosystemteknik
- Cretich, M. (author)
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- Haraldsson, Tommy (author)
- KTH,Mikrosystemteknik
- show more...
- Sola, L. (author)
- Bagnati, M. (author)
- Chiari, M. (author)
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- (creator_code:org_t)
- Chemical and Biological Microsystems Society, 2011
- 2011
- English.
- In: The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2011). - : Chemical and Biological Microsystems Society. - 9781618395955 ; , s. 311-313
- Conference paper (peer-reviewed)
Abstract
Subject headings
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- We present a one-step, reversible, and biocompatible bonding method of a stiff patterned microfluidic "Biosticker", based on off-stoichiometry thiol-ene (OSTE) polymers [1], to state-of-the-art spotted microarray surfaces. The method aims at improving and simplifying the batch back-end processing of microarrays. We illustrate its ease of use in two applications: a high sensitivity flow-through protein assay; and a DNA-hybridization test. Read-out was performed in a standard highvolume array scanner, and showed excellent spot homogeneity and intensity. The Biosticker is aimed to be a plug-in for existing microarray platforms to enable faster protein assays and DNA hybridizations through mass transport optimization.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Elektroteknik och elektronik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)
Keyword
- microarrays
- lab-on-chip loc
- OSTE
- off-stoichiometry thiol-ene
- microfluidic packaging
- Electrical engineering, electronics and photonics
- Elektroteknik, elektronik och fotonik
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- kon (subject category)
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