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Toward a confocal s...
Toward a confocal subcellular atlas of the human proteome
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- Barbe, Laurent (author)
- KTH,Skolan för bioteknologi (BIO)
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- Lundberg, Emma (author)
- KTH,Proteomik
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- Oksvold, Per (author)
- KTH,Proteomik
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- Stenius, Anna (author)
- KTH,Skolan för bioteknologi (BIO)
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- Lewin, Erland (author)
- KTH,Cellens fysik
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- Björling, Erik (author)
- KTH,Skolan för bioteknologi (BIO)
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- Asplund, Anna (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- Pontén, Fredrik (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- Brismar, Hjalmar (author)
- Karolinska Institutet,KTH,Cellens fysik
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- Uhlén, Mathias (author)
- KTH,Proteomik
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- Andersson-Svahn, Helene (author)
- KTH,Proteomik
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(creator_code:org_t)
- 2008
- 2008
- English.
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In: Molecular and cellular proteomics. - 1535-9476 .- 1535-9484. ; 7:3, s. 499-508
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Information on protein localization on the subcellular level is important to map and characterize the proteome and to better understand cellular functions of proteins. Here we report on a pilot study of 466 proteins in three human cell lines aimed to allow large scale confocal microscopy analysis using protein-specific antibodies. Approximately 3000 high resolution images were generated, and more than 80% of the analyzed proteins could be classified in one or multiple subcellular compartment(s). The localizations of the proteins showed, in many cases, good agreement with the Gene Ontology localization prediction model. This is the first large scale antibody-based study to localize proteins into subcellular compartments using antibodies and confocal microscopy. The results suggest that this approach might be a valuable tool in conjunction with predictive models for protein localization.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Keyword
- HIGH-THROUGHPUT MICROSCOPY; FLUORESCENCE MICROSCOPY; AUTOMATED MICROSCOPY; MEMBRANE-PROTEINS; LOCALIZATION; CELLS; TISSUES
- Bioengineering
- Bioteknik
- MEDICINE
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Barbe, Laurent
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Lundberg, Emma
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Oksvold, Per
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Stenius, Anna
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Lewin, Erland
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Björling, Erik
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show more...
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Asplund, Anna
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Pontén, Fredrik
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Brismar, Hjalmar
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Uhlén, Mathias
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Andersson-Svahn, ...
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show less...
- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
- Articles in the publication
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Molecular and ce ...
- By the university
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Royal Institute of Technology
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Uppsala University
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Karolinska Institutet