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Study of Real-time Lectin–Carbohydrate Interactions on the Surface of a Quartz Crystal Microbalance
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- Pei, Zhichao (author)
- KTH,Kemi
- Andersson, Henrik (author)
- Aastrup, Teodor (author)
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- (creator_code:org_t)
- Elsevier BV, 2005
- 2005
- English.
- In: Biosensors & bioelectronics. - : Elsevier BV. - 0956-5663 .- 1873-4235. ; 21:1, s. 60-66
- Journal article (peer-reviewed)
Abstract
Subject headings
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- A quartz crystal microbalance (QCM) biosensor system for lectin-carbohydrate interactions has been developed. Yeast mannan was immobilised on polystyrene-coated quartz crystals, and interactions tested with the lectin concanavalin A (Con A). The biosensor could be easily operated, where mannan immobilisation and all binding analyses were performed in real-time using a flow-through system. The apparent binding constant for yeast mannan to Con A was estimated to be 0.4 μM, well in accordance to reported literature values. In addition, the effective concentration values (EC50-values) for a series of mannose/mannoside ligands, acting as competitors to the mannan/Con A interaction, were determined to range from 0.18 to 5.3 mM, in good correlation with a related enzyme-labelled lectin assay (ELLA) protocol
Subject headings
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Keyword
- Carbohydrates
- Glycochemistry
- Lectins; Molecular recognition
- Quartz crystal microbalance
- Organic chemistry
- Organisk kemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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