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Altering the specif...
Altering the specificity of subtilisin B. lentus by combining site-directed mutagenesis and chemical modification
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- Berglund, P. (author)
- University of Toronto, Canada
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Stabile, M. R. (author)
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Gold, M. (author)
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Jones, J. B. (author)
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Mitchinson, C. (author)
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Bott, R. R. (author)
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Graycar, T. P. (author)
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(creator_code:org_t)
- 1996
- 1996
- English.
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In: Bioorganic & Medicinal Chemistry Letters. - 0960-894X .- 1464-3405. ; 6:21, s. 2507-2512
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The thiol side chain of the M222C mutant of the subtilisin from Bacillus lentus (SBL) has been chemically modified by methyl-, aminoethyl-, and sulfonatoethylthiosulfonate reagents. Introduction of charged residues into the active site of the enzyme reduced the catalytic efficiency with Suc-AAPF-pNA as the substrate, but resulted in better binding of sterically demanding boronic acid inhibitors.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Keyword
- boronic acid derivative
- subtilisin
- article
- Eggerthella lenta
- enzyme activity
- enzyme modification
- site directed mutagenesis
- Bacillus lentus
Publication and Content Type
- ref (subject category)
- art (subject category)
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