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3D-correlation-aver...
3D-correlation-averaging for membrane-protein-crystals
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- Koeck, Philip J. B. (author)
- Karolinska Institutet
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Purhonen, P. (author)
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Alvang, R. (author)
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Grundberg, B. (author)
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- Hebert, Hans (author)
- KTH,Strukturell bioteknik
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(creator_code:org_t)
- Berlin, Heidelberg : Springer Berlin Heidelberg, 2008
- 2008
- English.
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In: EMC 2008 14th European Microscopy Congress. - Berlin, Heidelberg : Springer Berlin Heidelberg. ; , s. 55-56
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Few 2-dimensional protein crystals can be used to determine high-resolution structures, whereas most electron crystallography projects remain at a resolution around 10 Ångström. This might be partly due to lack of flatness of many two-dimensional crystals [1]. We have investigated this problem and suggest single particle projection matching (3D-correlation averaging) of locally averaged unit cells to improve the quality of three-dimensional maps. Theoretical considerations and tests on simulated data demonstrate the feasibility of this refinement method [2].
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