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Photogenerated lect...
Photogenerated lectin sensors produced by thiol-ene/yne photo-click chemistry in aqueous solution
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- Norberg, Oscar, 1982- (author)
- KTH,Organisk kemi
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- Lee, Irene H. (author)
- Department of Chemistry, University of Massachusetts
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- Aastrup, Teodor (author)
- Attana AB
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- Yan, Mingdi (author)
- KTH,Organisk kemi
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- Ramström, Olof (author)
- KTH,Organisk kemi
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(creator_code:org_t)
- Elsevier BV, 2012
- 2012
- English.
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In: Biosensors & bioelectronics. - : Elsevier BV. - 0956-5663 .- 1873-4235. ; 34:1, s. 51-56
- Related links:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The photoinitiated radical reactions between thiols and alkenes/alkynes (thiol-ene and thiol-yne chemistry) have been applied to a functionalization methodology to produce carbohydrate-presenting surfaces for analyses of biomolecular interactions. Polymer-coated quartz surfaces were functionalized with alkenes or alkynes in a straightforward photochemical procedure utilizing perfluorophenylazide (PFPA) chemistry. The alkene/alkyne surfaces were subsequently allowed to react with carbohydrate thiols in water under UV-irradiation. The reaction can be carried out in a drop of water directly on the surface without photoinitiator, and any disulfide side products were easily washed away after the functionalization process. The resulting carbohydrate-presenting surfaces were evaluated in real-time studies of protein-carbohydrate interactions using a quartz crystal microbalance (QCM) flow-through system with recurring injections of selected lectins, with intermediate regeneration steps using low pH buffer. The resulting methodology proved fast, efficient and scalable to high-throughput analysis formats, and the produced surfaces showed significant protein binding with expected selectivities of the lectins used in the study.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- Photochemistry
- Click chemistry
- Thiol-ene/-yne
- Carbohydrates
- Lectins
- Quartz crystal microbalance
Publication and Content Type
- ref (subject category)
- art (subject category)
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