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Ultra-rapid melting...
Ultra-rapid melting curve analysis on beads for high-throughput genotyping of single nucleotide polymorphism
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- Russom, Aman (author)
- KTH,Mikrosystemteknik
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- Haasl, Sjoerd (author)
- KTH,Mikrosystemteknik
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Brookes, Anthony J. (author)
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- Andersson, Helene (author)
- KTH,Mikrosystemteknik
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- Stemme, Göran (author)
- KTH,Mikrosystemteknik
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(creator_code:org_t)
- Transducers Research Foundations, 2005
- 2005
- English.
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In: Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference. - : Transducers Research Foundations. - 9780974361116 ; , s. 1006-1008
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
Close
- This report describes a rapid solid-phase melting curve analysis method based on dynamic allele-specific hybridization (DASH) for single nucleotide polymorphism (SNP) genotyping. Beads with DNA duplexes are immobilized on the surface of a microheater chip. SNP on PCR products were scored, illustrating the sensitivity and robustness of the system. Single-bead detection and multiplexing were performed with a heating rate more than 20 times faster than conventional DASH. Hence, analyses that took more than 15 minutes could be performed in less than 1 minute, enabling ultra-rapid SNP analysis.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Annan teknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Other Engineering and Technologies (hsv//eng)
Keyword
- Beads
- DASH
- Microheater
- Single nucleotide polymorphism
Publication and Content Type
- ref (subject category)
- kon (subject category)
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