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Identification of A...
Identification of Autophagosome-associated Proteins and Regulators by Quantitative Proteomic Analysis and Genetic Screens
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Dengjel, Joern (author)
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Hoyer-Hansen, Maria (author)
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Nielsen, Maria O. (author)
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Eisenberg, Tobias (author)
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Harder, Lea M. (author)
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Schandorff, Soren (author)
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Farkas, Thomas (author)
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Kirkegaard, Thomas (author)
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Becker, Andrea C. (author)
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Schroeder, Sabrina (author)
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Vanselow, Katja (author)
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- Lundberg, Emma (author)
- KTH,Proteomik,Science for Life Laboratory, SciLifeLab
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Nielsen, Mogens M. (author)
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Kristensen, Anders R. (author)
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Akimov, Vyacheslav (author)
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Bunkenborg, Jakob (author)
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Madeo, Frank (author)
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Jaattela, Marja (author)
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Andersen, Jens S. (author)
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(creator_code:org_t)
- 2012
- 2012
- English.
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In: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 11:3
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Autophagy is one of the major intracellular catabolic pathways, but little is known about the composition of autophagosomes. To study the associated proteins, we isolated autophagosomes from human breast cancer cells using two different biochemical methods and three stimulus types: amino acid deprivation or rapamycin or concanamycin A treatment. The autophagosome- associated proteins were dependent on stimulus, but a core set of proteins was stimulus- independent. Remarkably, proteasomal proteins were abundant among the stimulus- independent common autophagosome- associated proteins, and the activation of autophagy significantly decreased the cellular proteasome level and activity supporting interplay between the two degradation pathways. A screen of yeast strains defective in the orthologs of the human genes encoding for a common set of autophagosome- associated proteins revealed several regulators of autophagy, including subunits of the retromer complex. The combined spatiotemporal proteomic and genetic data sets presented here provide a basis for further characterization of autophagosome biogenesis and cargo selection.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Dengjel, Joern
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Hoyer-Hansen, Ma ...
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Nielsen, Maria O ...
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Eisenberg, Tobia ...
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Harder, Lea M.
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Schandorff, Sore ...
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show more...
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Farkas, Thomas
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Kirkegaard, Thom ...
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Becker, Andrea C ...
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Schroeder, Sabri ...
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Vanselow, Katja
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Lundberg, Emma
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Nielsen, Mogens ...
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Kristensen, Ande ...
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Akimov, Vyachesl ...
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Bunkenborg, Jako ...
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Madeo, Frank
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Jaattela, Marja
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Andersen, Jens S ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Molecular & Cell ...
- By the university
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Royal Institute of Technology