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Dopaminergic neuron...
Dopaminergic neurons derived from BG01V2 : a variant of human embryonic stem cell line BG01
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- Vazin, Tandis (author)
- KTH,Genteknologi
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- Chen, Jia (author)
- Natl Inst Drug Abuse, Dev & Plast Sect, Cellular Neurobiol Res Branch, Intramural Res Program,NIH,Dept Hlth & Human Serv, Baltimore, MD
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- Spivak, Charles E. (author)
- Natl Inst Drug Abuse, Cellular Neurophysiol Sect, Cellular Neurobiol Res Branch, Intramural Res Program,NIH,Dept Hlth & Human Serv, Baltimore, MD
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- Amable, Rose (author)
- Natl Inst Drug Abuse, Dev & Plast Sect, Cellular Neurobiol Res Branch, Intramural Res Program,NIH,Dept Hlth & Human Serv, Baltimore, MD
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- Gabitzsch, Emily (author)
- Natl Inst Drug Abuse, Dev & Plast Sect, Cellular Neurobiol Res Branch, Intramural Res Program,NIH,Dept Hlth & Human Serv, Baltimore, MD
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- Lee, Chun-Ting (author)
- Natl Inst Drug Abuse, Dev & Plast Sect, Cellular Neurobiol Res Branch, Intramural Res Program,NIH,Dept Hlth & Human Serv, Baltimore, MD
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- Lupica, Carl R. (author)
- Natl Inst Drug Abuse, Cellular Neurophysiol Sect, Cellular Neurobiol Res Branch, Intramural Res Program,NIH,Dept Hlth & Human Serv, Baltimore, MD
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- Freed, William J. (author)
- Natl Inst Drug Abuse, Dev & Plast Sect, Cellular Neurobiol Res Branch, Intramural Res Program,NIH,Dept Hlth & Human Serv, Baltimore, MD
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(creator_code:org_t)
- 2008
- 2008
- English.
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In: Restorative Neurology and Neuroscience. - 0922-6028 .- 1878-3627. ; 26:6, s. 447-458
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Abstract
Subject headings
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- Background and Purpose: Human embryonic stem cells (hESC) areconsidered a renewable source of dopamine producing neurons, and are of particularinterest for their potential clinical use in Parkinson’s disease. In this study, wecharacterized human dopaminergic neurons generated by stromal-derived inducingactivity (SDIA) from BG01V2, a strain of human embryonic stem cell line, BG01,characterized by a chromosome 17 trisomy. Similar chromosomal changes have beenrepeatedly observed in hESC cultures in different laboratories, indicating the importanceof chromosome 17 for growth and adaptation of hESC to culture. Methods: Weinvestigated in vitro proliferation of differentiating cells using a BrDU incorporationassay, and monitored the cell population in long term cultures. Despite the cytogeneticabnormality, TH+ neurons were postmitotic at all stages of differentiation. After 30 daysof differentiation, cell division ceased in 91% of the overall population of cells in theculture, indicating intact cell cycle regulation. Results: Expression of midbrain specificmarker genes (Otx2, Pax5, Msx-1) showed differentiation of hESC-derived neuralprogenitor cells into midbrain specific dopamine neurons. These neurons expressed thedopamine transporter (DAT), and displayed functional DAT activity and electricalexcitability. Conclusions: TH+ cells derived from the BG01V2 hESC line using SDIAare postmitotic and have functional characteristics of normal dopaminergic neurons.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- SDIA
- embryonic stem cells
- human
- dopaminergic neuron
- PA6 cells
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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