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- Cassimjee, Karim EngelmarkKTH,Biokemi (author)
Chromobacterium violaceum omega-transaminase variant Trp60Cys shows increased specificity for (S)-1-phenylethylamine and 4 '-substituted acetophenones, and follows Swain-Lupton parameterisation
- Article/chapterEnglish2012
Publisher, publication year, extent ...
- Royal Society of Chemistry (RSC),2012
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:kth-99250
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-99250URI
- https://doi.org/10.1039/c2ob25893eDOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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Notes
- QC 20120724
- For biocatalytic production of pharmaceutically important chiral amines the.-transaminase enzymes have proven useful. Engineering of these enzymes has to some extent been accomplished by rational design, but mostly by directed evolution. By use of a homology model a key point mutation in Chromobacterium violaceum omega-transaminase was found upon comparison with engineered variants from homologous enzymes. The variant Trp60Cys gave increased specificity for (S)-1-phenylethylamine (29-fold) and 4'-substituted acetophenones (similar to 5-fold). To further study the effect of the mutation the reaction rates were Swain-Lupton parameterised. On comparison with the wild type, reactions of the variant showed increased resonance dependence; this observation together with changed pH optimum and cofactor dependence suggests an altered reaction mechanism.
Subject headings and genre
- NATURVETENSKAP Kemi Organisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Organic Chemistry hsv//eng
- OPTICALLY-ACTIVE AMINES; ASYMMETRIC-SYNTHESIS; CHIRAL AMINES; SUBSTRATE-SPECIFICITY; RESONANCE COMPONENTS; CHEMICAL-REACTIVITY; AMINOTRANSFERASE; SUBSTITUENT; IDENTIFICATION; BIOCATALYSIS
Added entries (persons, corporate bodies, meetings, titles ...)
- Humble, Maria SvedendahlKTH,Biokemi(Swepub:kth)u1mmbr9s (author)
- Land, HenrikKTH,Biokemi(Swepub:kth)u13t7x1r (author)
- Abedi, Vahak (author)
- Berglund, PerKTH,Biokemi(Swepub:kth)u1271chh (author)
- KTHBiokemi (creator_code:org_t)
Related titles
- In:Organic and biomolecular chemistry: Royal Society of Chemistry (RSC)10:28, s. 5466-54701477-05201477-0539
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