Search: onr:"swepub:oai:DiVA.org:liu-105899" >
A Voltage Dependent...
A Voltage Dependent Non-Inactivating Na+ Channel Activated during Apoptosis in Xenopus Oocytes
-
- Englund, Ulrika (author)
- Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet
-
- Gertow, Jens (author)
- Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet
-
- Kågedal, Katarina (author)
- Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet
-
show more...
-
- Elinder, Fredrik (author)
- Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet
-
show less...
-
(creator_code:org_t)
- 2014-02-28
- 2014
- English.
-
In: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 9:2, s. 0088381-
- Related links:
-
https://liu.diva-por... (primary) (Raw object)
-
show more...
-
https://journals.plo...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Ion channels in the plasma membrane are important for the apoptotic process. Different types of voltage-gated ion channels are up-regulated early in the apoptotic process and block of these channels prevents or delays apoptosis. In the present investigation we examined whether ion channels are up-regulated in oocytes from the frog Xenopus laevis during apoptosis. The two-electrode voltage-clamp technique was used to record endogenous ion currents in the oocytes. During staurosporine-induced apoptosis a voltage-dependent Na+ current increased three-fold. This current was activated at voltages more positive than 0 mV (midpoint of the open-probability curve was +55 mV) and showed almost no sign of inactivation during a 1-s pulse. The current was resistant to the Na+-channel blockers tetrodotoxin (1 mM) and amiloride (10 mM), while the Ca2+-channel blocker verapamil (50 mM) in the bath solution completely blocked the current. The intracellular Na+ concentration increased in staurosporine-treated oocytes, but could be prevented by replacing extracellular Na+ whith either K+ or Choline(+). Prevention of this influx of Na+ also prevented the STS-induced up-regulation of the caspase-3 activity, suggesting that the intracellular Na+ increase is required to induce apoptosis. Taken together, we have found that a voltage dependent Na+ channel is up-regulated during apoptosis and that influx of Na+ is a crucial step in the apoptotic process in Xenopus oocytes.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
PLOS ONE
(Search for host publication in LIBRIS)
To the university's database