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A miniaturized nanobiosensor for choline analysis
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- Pal, Souvik (author)
- BITS, Goa, India
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- Kumar Sharma, Manoj (author)
- Indian Institute of Technology Delhi, India
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- Danielsson, Bengt (author)
- Acromed Invest AB, Lund, Sweden
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- Elsevier, 2014
- 2014
- English.
- In: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 54, s. 558-564
- Journal article (peer-reviewed)
Abstract
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- A novel reusable chemiluminescence choline nanobiosensor has been developed using aligned zinc oxide nanorod-films (ZnONR). The chemically fashioned ZnONR were synthesized by hybrid wet chemical route onto glass substrates and used to fabricate a stable chemiluminescent choline biosensor. The biosensor was constructed by co-immobilization of the enzymes choline oxidase and peroxidase. The covalent immobilization of the enzymes on the ZnONR was achieved using 16-phosphonohexadecanoic acid as a cross-linker. The phosphonation of the ZnONR imparted significant stability to the immobilized enzyme as against physisorbed enzyme. A lower value of Michaelis-Menten constant (K-m), of 0.062 mM for the covalently coupled enzyme over the physisorbed enzymes facilitated enhanced stability of ZnONR nanobiosensor. The ZnONR-choline biosensor has been investigated over a wide range of choline from 0.0005 mM to 2 mM. Importantly, the recovery of choline in milk samples was close to 99%. Using the developed biosensor, choline was measurable even after 30 days with 60 repeated measurements proving the stability of the sensor (Intraday RSD%=2.83 and Interday RSD%=3.51).
Keyword
- Choline; Nanobiosensor; Zinc oxide nanorod; Chemiluminescence; Phosphonation; Milk
- TECHNOLOGY
- TEKNIKVETENSKAP
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- ref (subject category)
- art (subject category)
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