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Hybrid Nanoparticle...
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Aili, DanielImperial College London, U.K.
(author)
Hybrid Nanoparticle-Liposome Detection of Phospholipase Activity
- Article/chapterEnglish2011
Publisher, publication year, extent ...
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2010-08-26
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American Chemical Society (ACS),2011
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:liu-107949
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-107949URI
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https://doi.org/10.1021/nl1024062DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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A flexible nanoparticle-based phospholipase (PL) assay is demonstrated in which the enzymatic substrate is decoupled from the nanoparticle surface. Liposomes are loaded with a polypeptide that is designed to heteroassociate with a second polypeptide immobilized on gold nanoparticies. Release of this polypeptide from the liposornes, triggered by PL, induces a folding-dependent nanoparticle bridging aggregation. The colorimetric response from this aggregation enables straightforward and continuous detection of PL in the picomolar range. The speed, specificity, and flexibility of this assay make it appropriate for a range of applications, from point of care diagnostics to high throughput pharmaceutical screening.
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Mager, MImperial College London, U.K.
(author)
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Roche, DavidImperial College London, U.K.
(author)
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Stevens, MollyImperial College London, U.K.
(author)
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Imperial College London, U.K.
(creator_code:org_t)
Related titles
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In:Nano letters (Print): American Chemical Society (ACS)11:4, s. 1401-14051530-69841530-6992
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