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Medium-high resolut...
Medium-high resolution electrochemical genotyping of HLA-DQ2/DQ8 for detection of predisposition to coeliac disease
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- Joda, Hamdi (author)
- Universitat Rovira i Virgili, Tarragona, Spain
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- Beni, Valerio (author)
- Linköpings universitet,Biosensorer och bioelektronik,Tekniska högskolan,Universitat Rovira i Virgili, Tarragona, Spain
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- Alakkulpi, Noora (author)
- Finnish Red Cross Blood Service, Helsinki, Finland
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- Partanen, Jukka (author)
- Finnish Red Cross Blood Service, Helsinki, Finland
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- Lind, Kristina (author)
- TATAA Biocenter AB, Göteborg, Sweden
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- Strömbom, Linda (author)
- TATAA Biocenter AB, Göteborg, Sweden
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- Latta, Daniel (author)
- Institut für Mikrotechnik Mainz GmbH, Germany
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- Höth, Julian (author)
- Institut für Mikrotechnik Mainz GmbH, Germany
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- Katakis, Ioanis (author)
- Universitat Rovira i Virgili, Tarragona, Spain
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- O´Sullivan, Ciara K. (author)
- Universitat Rovira i Virgili, Tarragona, Spain; Institucio Catalana de Recerca i Estudis Avançats, Barcelona, Spain
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(creator_code:org_t)
- 2014-03-15
- 2014
- English.
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In: Analytical and Bioanalytical Chemistry. - : Springer Berlin/Heidelberg. - 1618-2642 .- 1618-2650. ; 406:12, s. 2757-2769
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Coeliac disease is a small intestinal disorder, induced by ingestion of gluten in genetically predisposed individuals. Coeliac disease has been strongly linked to human leukocyte antigens (HLA) located on chromosome 6, with almost 100 % of coeliac disease sufferers carrying either a HLA-DQ2 or HLA-DQ8 heterodimer, with the majority carrying HLA-DQ2 encoded by the DQA1*05:01/05:05, DQB1*02:01/02:02 alleles, whereas the remaining carry the HLA-DQ8 encoded by the DQA1*03:01, DQB1*03:02 alleles. In this work, we present the development of a multiplex electrochemical genosensor array of 36 electrodes, housed within a dedicated microfluidic platform and using a total of 10 sequence-specific probes for rapid medium-high resolution HLA-DQ2/DQ8 genotyping. An evaluation of the selectivity of the designed probes was carried out with the target sequences and 44 potentially interfering alleles, including single base mismatch differentiations; good selectivity was demonstrated. The performance of the electrochemical genosensor array was validated, analyzing real human samples for the presence of HLA-DQ2/DQ8 alleles, and compared with those obtained using laboratory-based HLA typing, and an excellent correlation was obtained.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- Electrochemical genosensors
- Coeliac disease
- HLA typing
- Sequence specificoligonucleotide probes
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Joda, Hamdi
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Beni, Valerio
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Alakkulpi, Noora
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Partanen, Jukka
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Lind, Kristina
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Strömbom, Linda
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show more...
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Latta, Daniel
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Höth, Julian
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Katakis, Ioanis
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O´Sullivan, Ciar ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Analytical Chemi ...
- Articles in the publication
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Analytical and B ...
- By the university
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Linköping University