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NMR studies of eryt...
NMR studies of erythrocytes immobilized in agarose and alginate gels
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- Lundberg, Peter (author)
- Linköpings universitet,Avdelningen för radiologiska vetenskaper,Hälsouniversitetet,Centrum för medicinsk bildvetenskap och visualisering, CMIV,Region Östergötland, Radiofysikavdelningen US,Department of Biochemistry, University of Sydney, Sydney, New South Wales. 2006, Australia
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- Berners-Price, Susan J. (author)
- Division of Science and Technology, Griffith University, Nathan, Queensland, 4111, Australia
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- Roy, Sushmita (author)
- Department of Biochemistry, University of Sydney, Sydney, New South Wales. 2006, Australia
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- Kuchel, Philip W. (author)
- Department of Biochemistry, University of Sydney, Sydney, New South Wales. 2006, Australia
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(creator_code:org_t)
- John Wiley & Sons, 1992
- 1992
- English.
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In: Magnetic Resonance in Medicine. - : John Wiley & Sons. - 0740-3194 .- 1522-2594. ; 25:2, s. 273-288
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- 31P and 13C NMR were used to study the energy metabolism in perfused, human erythrocytes. The erythrocytes were immobilized in agarose threads, Ca- or Ba-alginate beads, and Ba-alginate-coated agarose threads. Erythrocytes were easily washed out from the agarose threads, but not from alginate-containing gels. Various small molecules, such as hypophosphite, dimethyl methylphosphonate, and methylphosphonate, were taken up from the perfusion medium in a normal manner. In addition, the 2,3-bisphosphoglycerate (2,3-DPG) chemical shifts were sensitive to the oxygen partial pressure suggesting that O2 molecules were diffusing through the gel and modifying the binding of 2,3-DPG to hemoglobin. A combination of inosine and pyruvate stimulated the synthesis of 2,3-DPG, but only if inorganic phosphate was present in the perfusion medium. Inosine only resulted in a dramatic rise in the intracellular sugarphosphate concentrations. Furthermore, [2-13C]glucose was converted to [2-13C]lactate by immobilized cells at a rate which was comparable to that in a control suspension. In summary, immobilization in Ba-alginate-coated agarose threads was an efficient way of trapping human erythrocytes for whole cell NMR investigations.
Subject headings
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Keyword
- Alginates
- Diphosphoglyceric Acids/*metabolism
- Energy Metabolism/drug effects/*physiology
- Erythrocytes/*metabolism
- Gels
- Humans
- Inosine/pharmacology
- Magnetic Resonance Spectroscopy/*methods
- Oxygen/physiology
- Partial Pressure
- Pyruvates/pharmacology
- Sepharose
Publication and Content Type
- ref (subject category)
- art (subject category)
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