Search: onr:"swepub:oai:DiVA.org:liu-121813" >
An imidazole functi...
-
Magnusson, KarinLinköpings universitet,Kemi,Tekniska högskolan
(author)
An imidazole functionalized pentameric thiophene displays different staining patterns in normal and malignant cells
- Article/chapterEnglish2015
Publisher, publication year, extent ...
-
2015-10-07
-
Frontiers Media S.A.2015
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:liu-121813
-
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-121813URI
-
https://doi.org/10.3389/fchem.2015.00058DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Vid tiden för disputation förelåg publikationen som manuskriptFunding agencies: Swedish Foundation for Strategic Research; GeCONil [POIG.02.03.01-24-099/13]; ERC from the European Research Council
-
Molecular tools for fluorescent imaging of cells and their components are vital for understanding the function and activity of cells. Here, we report an imidazole functionalized pentameric oligothiophene, p-HTIm, that can be utilized for fluorescent imaging of cells. p-HTIm fluorescence in normal cells appeared in a peripheral punctate pattern partially co-localized with lysosomes, whereas a one-sided perinuclear Golgi associated localization of the dye was observed in malignant cells. The uptake of p-HTIm was temperature dependent and the intracellular target was reached within 1 h after staining. The ability of p-HTIm to stain cells was reduced when the imidazole side chain was chemically altered, verifying that specific imidazole side-chain functionalities are necessary for achieving the observed cellular staining. Our findings confirm that properly functionalized oligothiophenes can be utilized as fluorescent tools for vital staining of cells and that the selectivity towards distinct intracellular targets are highly dependent on the side-chain functionalities along the conjugated thiophene backbone.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Appelqvist, HannaLinköpings universitet,Kemi,Tekniska fakulteten(Swepub:liu)hanmi48
(author)
-
Cieślar-Pobuda, ArturLinköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten(Swepub:liu)artci82
(author)
-
Bäck, MarcusLinköpings universitet,Kemi,Tekniska högskolan(Swepub:liu)marba90
(author)
-
Kågedal, BertilLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten(Swepub:liu)berka70
(author)
-
Jonasson, JonLinköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,Region Östergötland, Klinisk patologi och klinisk genetik(Swepub:liu)jonjo59
(author)
-
Los, Marek J.Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten(Swepub:liu)marlo
(author)
-
Nilsson, Peter R.Linköpings universitet,Kemi,Tekniska högskolan(Swepub:liu)petni61
(author)
-
Linköpings universitetKemi
(creator_code:org_t)
Related titles
-
In:Frontiers in Chemistry: Frontiers Media S.A.32296-2646
Internet link
Find in a library
To the university's database