Orexin A Phosphorylates the gamma-Aminobutyric Acid Type A Receptor beta(2) Subunit on a Serine Residue and Changes the Surface Expression of the Receptor in SH-SY5Y Cells Exposed to Propofol

Andersson, HenrikÖstergötlands Läns Landsting,Linköpings universitet,Medicinska fakulteten,Anestesi- och intensivvårdskliniken US,Avdelningen för läkemedelsforskning (author)

Funding Agencies|County Council of Ostergotland ALF Grants; Linkoping University Hospital; Ella and Henry Stahl Research Foundation
Propofol activates the gamma-aminobutyric acid type A receptor (GABA(A)R) and causes a reversible neurite retraction, leaving a thin, thread-like structure behind; it also reverses the transport of vesicles in rat cortical neurons. The awakening peptide orexin A (OA) inhibits this retraction via phospholipase D (PLD) and protein kinase CE (PKCE). The human SH-SY5Y cells express both GABA(A)Rs and orexin 1 and 2 receptors. These cells are used to examine the interaction between OA and the GABAAR. The effects of OA are studied with flow cytometry and immunoblotting. This study shows that OA stimulates phosphorylation on the serine residues of the GABA(A)R beta(2) subunit and that the phosphorylation is caused by the activation of PLD and PKCE. OA administration followed by propofol reduces the cell surface expression of the GABA(A)R, whereas propofol stimulation before OA increases the surface expression. The GABA(A)R beta(2) subunit is important for receptor recirculation, and the effect of OA on propofol-stimulated cells may be due to a disturbed recirculation of the GABA(A)R. (C) 2015 Wiley Periodicals, Inc.

Björnström-Karlsson, KarinLinköpings universitet,Avdelningen för läkemedelsforskning,Medicinska fakulteten,Region Östergötland, ANOPIVA US(Swepub:liu)karbj69 (author)
Eintrei, ChristinaLinköpings universitet,Avdelningen för läkemedelsforskning,Medicinska fakulteten,Region Östergötland, ANOPIVA US(Swepub:liu)chrei93 (author)
Sundqvist, TommyLinköpings universitet,Avdelningen för kliniska vetenskaper,Medicinska fakulteten(Swepub:liu)tomsu30 (author)
Linköpings universitetMedicinska fakulteten (creator_code:org_t)

In:Journal of Neuroscience Research: WILEY-BLACKWELL93:11, s. 1748-17550360-40121097-4547

By the author/editor: Andersson, Henri ...; Björnström-Karls ...; Eintrei, Christi ...; Sundqvist, Tommy

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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