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DFL23448, A Novel T...
DFL23448, A Novel Transient Receptor Potential Melastin 8-Selective Ion Channel Antagonist, Modifies Bladder Function and Reduces Bladder Overactivity in Awake Rats
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- Mistretta, Francesco A. (author)
- IRCCS Osped San Raffaele, Italy; Lund University, Sweden
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- Russo, Andrea (author)
- IRCCS Osped San Raffaele, Italy
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- Castiglione, Fabio (author)
- IRCCS Osped San Raffaele, Italy
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- Bettiga, Arianna (author)
- IRCCS Osped San Raffaele, Italy
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- Colciago, Giorgia (author)
- IRCCS Osped San Raffaele, Italy
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- Montorsi, Francesco (author)
- IRCCS Osped San Raffaele, Italy; University of Vita Salute San Raffaele, Italy
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- Brandolini, Laura (author)
- Research Centre Dompe Farmaceutici SpA, Italy
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- Aramini, Andrea (author)
- Research Centre Dompe Farmaceutici SpA, Italy
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- Bianchini, Gianluca (author)
- Research Centre Dompe Farmaceutici SpA, Italy
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- Allegretti, Marcello (author)
- Research Centre Dompe Farmaceutici SpA, Italy
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- Bovolenta, Silvia (author)
- Axxam, Italy
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- Russo, Roberto (author)
- University of Naples Federico II, Italy
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- Benigni, Fabio (author)
- IRCCS Osped San Raffaele, Italy
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- Hedlund, Petter (author)
- Linköpings universitet,Avdelningen för läkemedelsforskning,Medicinska fakulteten,Region Östergötland, Klinisk farmakologi,IRCCS Osped San Raffaele, Italy; Lund University, Sweden
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(creator_code:org_t)
- 2015-11-06
- 2016
- English.
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In: Journal of Pharmacology and Experimental Therapeutics. - : AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS. - 0022-3565 .- 1521-0103. ; 356:1, s. 200-211
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https://jpet.aspetjo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- The transient receptor potential melastin 8 ion channel (TRPM8) is implicated in bladder sensing but limited information on TRPM8 antagonists in bladder overactivity is available. This study characterizes a new TRPM8-selective antagonist (DFL23448 [5-(2-ethyl-2H-tetrazol-5-yl)-2-(3-fluorophenyl)-1,3-thiazol-4-ol]) and evaluates it in cold-induced behavioral tests and tests on bladder function and experimental bladder overactivity in vivo in rats. DFL23448 displayed IC50 values of 10 and 21 nM in hTRPM8 human embryonic kidney 293 cells activated by Cooling Agent 10 or cold, but it had limited activity (IC50 > 10 mu M) at transient receptor potential vanilloids TRPV1, TRPA1, or TRPV4 or at various G protein-coupled receptors. In rats, DFL23448 administered intravenously or orally had a half-life of 37 minutes or 4.9 hours, respectively. DLF23448 (10 mg/kg i.v.) reduced icilin-induced "wet dog-like" shakes in rats. Intravesical DFL23448 (10 mg/l), but not vehicle, increased micturition intervals, micturition volume, and bladder capacity. During bladder overactivity by intravesical prostaglandin E-2 (PGE(2)), vehicle controls exhibited reductions in micturition intervals, micturition volumes, and bladder capacity by 37%-39%, whereas the same parameters only decreased by 12%-15% (P < 0.05-0.01 versus vehicle) in DFL23448-treated rats. In vehicle-treated rats, but not in DFL23448-treated rats, intravesical PGE(2) increased bladder pressures. Intravenous DFL23448 at 10 mg/kg, but not 1 mg/kg DFL23448 or vehicle, increased micturition intervals, micturition volumes, and bladder capacity. During bladder overactivity by intravesical PGE(2), micturition intervals, micturition volumes, and bladder capacity decreased in vehicle- and 1 mg/kg DFL23448-treated rats, but not in 10 mg/kg DFL23448-treated rats. Bladder pressures increased less in rats treated with DFL23448 10 mg/kg than in vehicle-or 1 mg/kg DFL23448-treated rats. DFL23448 (10 mg/kg i.v.), but not vehicle, prevented cold stress-induced bladder overactivity. Our results support a role for bladder TRPM8-mediated signals in experimental bladder overactivity.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
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- ref (subject category)
- art (subject category)
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Mistretta, Franc ...
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Russo, Andrea
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Castiglione, Fab ...
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Bettiga, Arianna
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Colciago, Giorgi ...
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Montorsi, France ...
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Brandolini, Laur ...
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Aramini, Andrea
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Bianchini, Gianl ...
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Allegretti, Marc ...
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Bovolenta, Silvi ...
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Russo, Roberto
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Benigni, Fabio
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Hedlund, Petter
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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Journal of Pharm ...
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Linköping University