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Depletion of Intrac...
Depletion of Intracellular Thiols and Increased Production of 4-Hydroxynonenal that Occur During Cryopreservation of Stallion Spermatozoa Lead to Caspase Activation, Loss of Motility, and Cell Death
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- Martin Munoz, Patricia (author)
- University of Extremadura, Spain
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- Ortega Ferrusola, Cristina (author)
- University of Extremadura, Spain
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- Vizuete, Guillermo (author)
- University of Extremadura, Spain
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- Plaza Davila, Maria (author)
- University of Extremadura, Spain
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- Rodriguez-Martinez, Heriberto (author)
- Linköpings universitet,Avdelningen för kliniska vetenskaper,Medicinska fakulteten
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- Pena, Fernando J. (author)
- University of Extremadura, Spain
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(creator_code:org_t)
- SOC STUDY REPRODUCTION, 2015
- 2015
- English.
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In: Biology of Reproduction. - : SOC STUDY REPRODUCTION. - 0006-3363 .- 1529-7268. ; 93:6, s. 143-
- Related links:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Oxidative stress has been linked to sperm death and the accelerated senescence of cryopreserved spermatozoa. However, the molecular mechanisms behind this phenomenon remain poorly understood. Reactive oxygen species (ROS) are considered relevant signaling molecules for sperm function, only becoming detrimental when ROS homeostasis is lost. We hereby hypothesize that a major component of the alteration of ROS homeostasis in cryopreserved spermatozoa is the exhaustion of intrinsic antioxidant defense mechanisms. To test this hypothesis, semen from seven stallions was frozen using a standard technique. The parameters of sperm quality (motility, velocity, and membrane integrity) and markers of sperm senescence (caspase 3, 4-hydroxynonenal, and mitochondrial membrane potential) were assessed before and after cryopreservation. Changes in the intracellular thiol content were also monitored. Cryopreservation caused significant increases in senescence markers as well as dramatic depletion of intracellular thiols to less than half of the initial values (P < 0.001) postthaw. Interestingly, very high and positive correlations were observed among thiol levels with sperm functionality postthaw: total motility (r = 0.931, P < 0.001), progressive motility (r = 0.904, P < 0.001), and percentage of live spermatozoa without active caspase 3 (r = 0.996, P < 0.001). In contrast, negative correlations were detected between active caspase 3 and thiol content both in living (r = -0.896) and dead (r = -0.940) spermatozoa; additionally, 4-hydroxynonenal levels were negatively correlated with thiol levels (r = -0.856). In conclusion, sperm functionality postthaw correlates with the maintenance of adequate levels of intracellular thiols. The accelerated senescence of thawed spermatozoa is related to oxidative and electrophilic stress induced by increased production of 4-hydroxynoneal in thawed samples once intracellular thiols are depleted.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Keyword
- cryopreservation; equids (horses
- donkeys
- zebras); flow cytometry; ROS; semen; sperm; sperm senescence; spermatozoa; stallion; thiols
Publication and Content Type
- ref (subject category)
- art (subject category)
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