The relationship between ram sperm head morphometry and fertility depends on the procedures of acquisition and analysis used.

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MARC

de Paz, PaulinoDepartment of Molecular Biology, University of León, León, Spain (author)

The relationship between ram sperm head morphometry and fertility depends on the procedures of acquisition and analysis used.

Article/chapterEnglish2011

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Elsevier,2011
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LIBRIS-ID:oai:DiVA.org:liu-130195
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-130195URI
https://doi.org/10.1016/j.theriogenology.2011.05.038DOI

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Language:English
Summary in:English

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Sperm head morphometry is a parameter in the evaluation of semen that has been associated with fertility in two ways: comparing morphometric measures between predefined groups of fertility; or analyzing morphometric data by multivariate techniques to identify cell populations. We analyzed the morphometry of ram sperm head by three procedures and checked its relationship with male fertility. A Computer-Aided Sperm Morphometric Assessment procedure (CASMA), an image analysis software (NIS-Elements) in combination with an optical microscope (MO-NIS) and this image analysis software in combination with a scanning electron microscope (SEM-NIS) were used. Eight morphometric parameters were assessed: length, width, area, perimeter, ellipticity, form factor, elongation and regularity. We observed significant differences between the morphometric data of sperm head obtained with three study procedures. The CASMA procedure shows the highest values for all parameters and the SEM-NIS procedure the lowest. The analysis of a semen sample, when only the mean of morphometric parameters is used to describe the cell population, is too limited to interpret their fertilizing capacity. It is essential to analyze the complex structure of the samples by defining subpopulations by multivariate methods. With few exceptions, the means of each morphometric parameter differ between the three subpopulations analyzed in each procedure. Only the subpopulations obtained with the MO-NIS procedure showed a significant correlation with male fertility. In short, it is necessary to establish an instrumental standard for the analysis of sperm morphometry to obtain reliable results and we believe that the MO-NIS system presents these basic requirements.

Subject headings and genre

NATURVETENSKAP Biologi Utvecklingsbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Developmental Biology hsv//eng
Ram
Sperm
Head morphometry
Cell subpopulations
Fertility

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Mata-Campuzano, MaríaDepartment of Molecular Biology, University of León, León, Spain (author)
Tizado, Emilio JorgeDepartment of Biodiversity and Environmental Management, University of León, León, Spain (author)
Alvarez, MercedesDepartment of Veterinary Medicine, Anatomy and Surgery, University of León, León, Spain (author)
Alvarez-Rodríguez, ManuelDepartment of Veterinary Medicine, Anatomy and Surgery, University of León, León, Spain(Swepub:liu)manal80 (author)
Herraez, PazDepartment of Molecular Biology, University of León, León, Spain (author)
Anel, LuisDepartment of Veterinary Medicine, Anatomy and Surgery, University of León, León, Spain (author)
Department of Molecular Biology, University of León, León, SpainDepartment of Biodiversity and Environmental Management, University of León, León, Spain (creator_code:org_t)

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In:Theriogenology: Elsevier76:7, s. 1313-13250093-691X1879-3231

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Developmental Bi ...

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By the university: Linköping University

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