Probes and techniques for sperm evaluation by flow cytometry

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Fältnamn	Indikatorer	Metadata
000		03103naa a2200361 4500
001		oai:DiVA.org:liu-130198
003		SwePub
008		160714s2010 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1301982 URI
024	7	a https://doi.org/10.1111/j.1439-0531.2010.01622.x2 DOI
040		a (SwePub)liu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Martínez-Pastor, Felipeu ITRA-ULE, INDEGSAL, Departments of Molecular Biology (Cell Biology), University of León, León, Spain4 aut
245	1 0	a Probes and techniques for sperm evaluation by flow cytometry
264		c 2010-05-17
264	1	b Blackwell Verlag,c 2010
338		a print2 rdacarrier
520		a CONTENTS: Flow cytometry has become an important technique in sperm evaluation and is increasingly used both for routine assessment and for research in veterinary science. We have revised the literature, describing fluorescent probes that have been used for analysing spermatozoa by flow cytometry, regarding: viability, acrosomal status, capacitation, mitochondrial status, apoptotic markers, oxidative stress markers, DNA damage, sperm counting and sperm sizing. Details and problems of some techniques are reviewed, with special attention to the occurrence of non-sperm particles in the samples ('debris'). New and promising aspects of flow cytometry, such as sperm sorting using viability markers as selection criteria, are highlighted. The relationship between flow cytometry analyses and fertility and their future improvements are considered.
650	7	a NATURVETENSKAPx Biologix Utvecklingsbiologi0 (SwePub)106142 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Developmental Biology0 (SwePub)106142 hsv//eng
700	1	a Mata-Campuzano, Mariau ITRA-ULE, INDEGSAL, Departments of Molecular Biology (Cell Biology), University of León, León, Spain4 aut
700	1	a Alvarez-Rodriguez, Manuelu ITRA-ULE, INDEGSAL, Animal Reproduction and Obstetrics, University of León, León, Spain4 aut0 (Swepub:liu)manal80
700	1	a Alvarez, Mercedesu ITRA-ULE, INDEGSAL, Animal Reproduction and Obstetrics, University of León, León, Spain4 aut
700	1	a Anel, Luisu ITRA-ULE, INDEGSAL, Animal Reproduction and Obstetrics, University of León, León, Spain4 aut
700	1	a de Paz, Paulinou ITRA-ULE, INDEGSAL, Departments of Molecular Biology (Cell Biology), University of León, León, Spain4 aut
710	2	a ITRA-ULE, INDEGSAL, Departments of Molecular Biology (Cell Biology), University of León, León, Spainb ITRA-ULE, INDEGSAL, Animal Reproduction and Obstetrics, University of León, León, Spain4 org
773	0	t Reproduction in domestic animalsd : Blackwell Verlagg 45 Suppl 2, s. 67-78q 45 Suppl 2<67-78x 0936-6768x 1439-0531
856	4	u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1439-0531.2010.01622.x
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-130198
856	4 8	u https://doi.org/10.1111/j.1439-0531.2010.01622.x

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