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Hybrid Microfluidic...
Hybrid Microfluidic Platform for Multifactorial Analysis Based on Electrical Impedance, Refractometry, Optical Absorption and Fluorescence
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- Pereira, Fabio M. (author)
- University of Nova Lisboa, Portugal; CEMOP UNINOVA, Portugal; University of Nova Lisboa, Portugal
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- Bernacka-Wojcik, Iwona (author)
- Linköpings universitet,Fysik och elektroteknik,Tekniska fakulteten,University of Nova Lisboa, Portugal; CEMOP UNINOVA, Portugal
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- Rodrigues Ribeiro, Rita S. (author)
- University of Porto, Portugal; Institute Syst and Comp Engn Technology and Science INESC TEC, Portugal
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- Teresa Lobato, Maria (author)
- University of Nova Lisboa, Portugal; CEMOP UNINOVA, Portugal
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- Fortunato, Elvira (author)
- University of Nova Lisboa, Portugal; CEMOP UNINOVA, Portugal
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- Martins, Rodrigo (author)
- University of Nova Lisboa, Portugal; CEMOP UNINOVA, Portugal
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- Igreja, Rui (author)
- University of Nova Lisboa, Portugal; CEMOP UNINOVA, Portugal
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- Jorge, Pedro A. S. (author)
- Institute Syst and Comp Engn Technology and Science INESC TEC, Portugal
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- Aguas, Hugo (author)
- University of Nova Lisboa, Portugal; CEMOP UNINOVA, Portugal
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- Martin Gonzalez Oliva, Abel (author)
- University of Nova Lisboa, Portugal
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(creator_code:org_t)
- 2016-10-07
- 2016
- English.
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In: Micromachines. - : MDPI AG. - 2072-666X. ; 7:10
- Related links:
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https://liu.diva-por... (primary) (Raw object)
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https://www.mdpi.com...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Subject headings
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- This paper describes the development of a novel microfluidic platform for multifactorial analysis integrating four label-free detection methods: electrical impedance, refractometry, optical absorption and fluorescence. We present the rationale for the design and the details of the microfabrication of this multifactorial hybrid microfluidic chip. The structure of the platform consists of a three-dimensionally patterned polydimethylsiloxane top part attached to a bottom SU-8 epoxy-based negative photoresist part, where microelectrodes and optical fibers are incorporated to enable impedance and optical analysis. As a proof of concept, the chip functions have been tested and explored, enabling a diversity of applications: (i) impedance-based identification of the size of micro beads, as well as counting and distinguishing of erythrocytes by their volume or membrane properties; (ii) simultaneous determination of the refractive index and optical absorption properties of solutions; and (iii) fluorescence-based bead counting.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Annan medicinteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Other Medical Engineering (hsv//eng)
Keyword
- single cell analysis; label-free methods; impedance spectroscopy; refractometry; optical absorption; hybrid microfluidic chip
Publication and Content Type
- ref (subject category)
- art (subject category)
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