Search: onr:"swepub:oai:DiVA.org:liu-133538" >
Wild-Type and Non-W...
Wild-Type and Non-Wild-Type Mycobacterium tuberculosis MIC Distributions for the Novel Fluoroquinolone Antofloxacin Compared with Those for Ofloxacin, Levofloxacin, and Moxifloxacin
-
- Yu, Xia (author)
- Capital Medical University, Peoples R China
-
- Wang, Guirong (author)
- Capital Medical University, Peoples R China
-
- Chen, Suting (author)
- Capital Medical University, Peoples R China
-
show more...
-
- Wei, Guomei (author)
- Capital Medical University, Peoples R China
-
- Shang, Yuanyuan (author)
- Capital Medical University, Peoples R China
-
- Dong, Lingling (author)
- Capital Medical University, Peoples R China
-
- Schön, Thomas (author)
- Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten,Kalmar County Hospital, Sweden
-
- Moradigaravand, Danesh (author)
- Wellcome Trust Sanger Institute, England
-
- Parkhill, Julian (author)
- Wellcome Trust Sanger Institute, England
-
- Peacock, Sharon J. (author)
- Wellcome Trust Sanger Institute, England; University of Cambridge, England; London School Hyg and Trop Med, England
-
- Koser, Claudio U. (author)
- University of Cambridge, England
-
- Huang, Hairong (author)
- Capital Medical University, Peoples R China
-
show less...
-
(creator_code:org_t)
- AMER SOC MICROBIOLOGY, 2016
- 2016
- English.
-
In: Antimicrobial Agents and Chemotherapy. - : AMER SOC MICROBIOLOGY. - 0066-4804 .- 1098-6596. ; 60:9, s. 5232-5237
- Related links:
-
https://liu.diva-por... (primary) (Raw object)
-
show more...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Antofloxacin (AFX) is a novel fluoroquinolone that has been approved in China for the treatment of infections caused by a variety of bacterial species. We investigated whether it could be repurposed for the treatment of tuberculosis by studying its in vitro activity. We determined the wild-type and non-wild-type MIC ranges for AFX as well as ofloxacin (OFX), levofloxacin (LFX), and moxifloxacin (MFX), using the microplate alamarBlue assay, of 126 clinical Mycobacterium tuberculosis strains from Beijing, China, of which 48 were OFX resistant on the basis of drug susceptibility testing on Lowenstein-Jensen medium. The MIC distributions were correlated with mutations in the quinolone resistance-determining regions of gyrA (Rv0006) and gyrB (Rv0005). Pharmacokinetic/pharmacodynamic (PK/PD) data for AFX were retrieved from the literature. AFX showed lower MIC levels than OFX but higher MIC levels than LFX and MFX on the basis of the tentative epidemiological cutoff values (ECOFFs) determined in this study. All strains with non-wild-type MICs for AFX harbored known resistance mutations that also resulted in non-wild-type MICs for LFX and MFX. Moreover, our data suggested that the current critical concentration of OFX for Lowenstein-Jensen medium that was recently revised by the World Health Organization might be too high, resulting in the misclassification of phenotypically non-wildtype strains with known resistance mutations as wild type. On the basis of our exploratory PK/PD calculations, the current dose of AFX is unlikely to be optimal for the treatment of tuberculosis, but higher doses could be effective.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Yu, Xia
-
Wang, Guirong
-
Chen, Suting
-
Wei, Guomei
-
Shang, Yuanyuan
-
Dong, Lingling
-
show more...
-
Schön, Thomas
-
Moradigaravand, ...
-
Parkhill, Julian
-
Peacock, Sharon ...
-
Koser, Claudio U ...
-
Huang, Hairong
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Infectious Medic ...
- Articles in the publication
-
Antimicrobial Ag ...
- By the university
-
Linköping University