Search: onr:"swepub:oai:DiVA.org:liu-13359" >
The binding of huma...
The binding of human Carbonic Anhydrase II by functionalized folded polypeptide receptors
-
- Andersson, Theresa (author)
- Linköpings universitet,Institutionen för fysik, kemi och biologi,Tekniska högskolan
-
- Lundqvist, Martin (author)
- Linköpings universitet,Molekylär Bioteknik,Tekniska högskolan
-
- Dolphin, Gunnar T. (author)
- Linköpings universitet,Institutionen för fysik, kemi och biologi,Tekniska högskolan
-
show more...
-
- Enander, Karin (author)
- Linköpings universitet,Sensorvetenskap och Molekylfysik,Tekniska högskolan
-
- Jonsson, Bengt-Harald (author)
- Linköpings universitet,Molekylär Bioteknik,Tekniska högskolan
-
- Nilsson, Jonas W. (author)
- Linköpings universitet,Organisk Kemi,Tekniska högskolan
-
- Baltzer, Lars (author)
- Linköpings universitet,Institutionen för fysik, kemi och biologi,Tekniska högskolan
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2005
- 2005
- English.
-
In: Chemistry and Biology. - : Elsevier BV. - 1074-5521 .- 1879-1301. ; 12:11, s. 1245-1252
- Related links:
-
http://urn.kb.se/res...
-
show more...
-
http://www.cell.com/...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Several receptors for human carbonic anhydrase II (HCAII) have been prepared by covalently attaching benzenesulfonamide carboxylates via aliphatic aminocarboxylic acid spacers of variable length to the side chain of a lysine residue in a designed 42 residue helix-loop-helix motif. The sulfonamide group binds to the active site zinc ion of human carbonic anhydrase II located in a 15 Å deep cleft. The dissociation constants of the receptor-HCAII complexes were found to be in the range from low micromolar to better than 20 nM, with the lowest affinities found for spacers with less than five methylene groups and the highest affinity found for the spacer with seven methylene groups. The results suggest that the binding is a cooperative event in which both the sulfonamide residue and the helix-loop-helix motif contribute to the overall affinity.
Keyword
- NATURAL SCIENCES
- NATURVETENSKAP
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database