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CD8+T cells of chro...
CD8+T cells of chronic HCV-infected patients express multiple negative immune checkpoints following stimulation with HCV peptides
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- Barathan, Muttiah (author)
- University of Malaya, Malaysia
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- Mohamed, Rosmawati (author)
- University of Malaya, Malaysia
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- Vadivelu, Jamuna (author)
- University of Malaya, Malaysia
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- Yen Chang, Li (author)
- University of Malaya, Malaysia
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- Vignesh, Ramachandran (author)
- University of Kuala Lumpur, Malaysia
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- Krishnan, Jayalakshmi (author)
- CUTN, India
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- Sigamani, Panneer (author)
- CUTN, India
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- Saeidi, Alireza (author)
- University of Malaya, Malaysia
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- Ravishankar Ram, M. (author)
- University of Malaya, Malaysia
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- Velu, Vijayakumar (author)
- Emory Vaccine Centre, GA 30329 USA
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- Larsson, Marie (author)
- Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten
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- Shankar, Esaki M. (author)
- University of Malaya, Malaysia; CUTN, India; University of Malaya, Malaysia
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(creator_code:org_t)
- ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017
- 2017
- English.
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In: Cellular Immunology. - : ACADEMIC PRESS INC ELSEVIER SCIENCE. - 0008-8749 .- 1090-2163. ; 313
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Hepatitis C virus (HCV)-specific CD4+ and CD8+ T cells are key to successful viral clearance in HCV disease. Accumulation of exhausted HCV-specific T cells during chronic infection results in considerable loss of protective functional immune responses. The role of T-cell exhaustion in chronic HCV disease remains poorly understood. Here, we studied the frequency of HCV peptide-stimulated T cells expressing negative immune checkpoints (PD-1, CTLA-4, TRAIL, TIM-3 and BTLA) by flow cytometry, and measured the levels of Th1/Th2/Th17 cytokines secreted by T cells by a commercial Multi-Analyte ELISArray (TM) following in vitro stimulation of T cells using HCV peptides and phytohemagglutinin (PHA). HCV peptide stimulated CD4+ and CD8+ T cells of chronic HCV (CHC) patients showed significant increase of CTLA-4. Furthermore, HCV peptide-stimulated CD4+ T cells of CHC patients also displayed relatively higher levels of PD-1 and TRAIL, whereas TIM-3 was up-regulated on HCV peptide-stimulated CD8+ T cells. Whereas the levels of IL-10 and TGF-beta 1 were significantly increased, the levels of pro-inflammatory cytokines IL-2, TNF-alpha, IL-17A and IL-6 were markedly decreased in the T cell cultures of CHC patients. Chronic HCV infection results in functional exhaustion of CD4+ and CD8+ T cells likely contributing to viral persistence. (C) 2016 Elsevier Inc. All rights reserved.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Co-inhibitory receptors; HCV; Immune checkpoint; PD-1; TIM-3; T-cell exhaustion
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Barathan, Muttia ...
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Mohamed, Rosmawa ...
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Vadivelu, Jamuna
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Yen Chang, Li
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Vignesh, Ramacha ...
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Krishnan, Jayala ...
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Sigamani, Pannee ...
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Saeidi, Alireza
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Ravishankar Ram, ...
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Velu, Vijayakuma ...
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Larsson, Marie
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Shankar, Esaki M ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
- Articles in the publication
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Cellular Immunol ...
- By the university
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Linköping University