onr:"swepub:oai:DiVA.org:liu-143332"
Search: onr:"swepub:oai:DiVA.org:liu-143332" > Noninvasive imaging...
- 1 of 1
- Previous record
- Next record
- To hitlist
Noninvasive imaging of Staphylococcus aureus infections with a nuclease-activated probe
-
- Hernandez, Frank J (author)
- Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of of Iowa, Iowa City, IA, United States
-
- Huang, Lingyan (author)
- Integrated DNA Technologies (IDT), Coralville, IA, United States
-
- Olson, Michael E. (author)
- Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of of Iowa, Iowa City, IA, United States
- show more...
- show less...
- Department of Internal Medicine, Roy J and Lucille A. Carver College of Medicine, University of of Iowa, Iowa City, IA, United States Integrated DNA Technologies (IDT), Coralville, IA, United States (creator_code:org_t)
- 2014-02-02
- 2014
- English.
- In: Nature Medicine. - : Nature Publishing Group. - 1078-8956 .- 1546-170X. ; 20:3, s. 301-306
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Technologies that enable the rapid detection and localization of bacterial infections in living animals could address an unmet need for infectious disease diagnostics. We describe a molecular imaging approach for the specific, noninvasive detection of S. aureus based on the activity of the S. aureus secreted nuclease, micrococcal nuclease (MN). Several short synthetic oligonucleotides, rendered resistant to mammalian serum nucleases by various chemical modifications and flanked with a fluorophore and quencher, were activated upon degradation by purified MN and in S. aureus culture supernatants. A probe consisting of a pair of deoxythymidines flanked by several 2′-O-methyl-modified nucleotides was activated in culture supernatants of S. aureus but not in culture supernatants of several other pathogenic bacteria. Systemic administration of this probe to mice bearing S. aureus muscle infections resulted in probe activation at the infection sites in an MN-dependent manner. This new bacterial imaging approach has potential clinical applicability for infections with S. aureus and several other medically important pathogens. © 2014 Nature America, Inc.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Nature Medicine (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Hernandez, Frank ...
- Huang, Lingyan
- Olson, Michael E ...
- Powers, Kristy M ...
- Hernandez, Luiza ...
- Meyerholz, David ...
- show more...
- Thedens, Daniel ...
- Behlke, Mark A.
- Horswill, Alexan ...
- Mcnamara II, Jam ...
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Microbiology in ...
- Articles in the publication
- Nature Medicine
- By the university
- Linköping University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
