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Studies of luminesc...
Studies of luminescent conjugated polythiophene derivatives-Enhanced spectral discrimination of protein conformational states
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- Åslund, Andreas (author)
- Linköpings universitet,Organisk Kemi,Tekniska högskolan
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- Herland, Anna (author)
- Linköpings universitet,Biomolekylär och Organisk Elektronik,Tekniska högskolan
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- Hammarström, Per (author)
- Linköpings universitet,Biokemi,Tekniska högskolan
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- Nilsson, Peter (author)
- Linköpings universitet,Organisk Kemi,Tekniska högskolan
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- Jonsson, Bengt-Harald (author)
- Linköpings universitet,Molekylär Bioteknik,Tekniska högskolan
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- Inganäs, Olle (author)
- Linköpings universitet,Biomolekylär och Organisk Elektronik,Tekniska högskolan
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- Konradsson, Peter (author)
- Linköpings universitet,Organisk Kemi,Tekniska högskolan
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(creator_code:org_t)
- 2007-10-17
- 2007
- English.
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In: Bioconjugate chemistry. - : American Chemical Society (ACS). - 1043-1802 .- 1520-4812. ; 18:6, s. 1860-1868
- Related links:
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http://urn.kb.se/res...
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http://urn.kb.se/res...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Improved probes for amyloid fibril formation are advantageous for the early detection and better understanding of this disease-associated process. Here, we report a comparative study of eight luminescent conjugated polythiophene derivates (LCPs) and their discrimination of a protein (insulin) in the native or amyloid-like fibrillar state. For two of the LCPs, the synthesis is reported. Compared to their monomer-based analogues, trimer-based LCPs showed significantly better optical signal specificity for amyloid-like fibrils, seen from increased quantum yield and spectral shift. The trimer-based LCPs alone were highly quenched and showed little interaction with native insulin, as seen from analytical ultracentrifugation and insignificant spectral differences from the trimer-based LCP in buffered and native protein solution. Hence, the trimer-based LCPs showed enhanced discrimination between the amyloid-like fibrillar state and the corresponding native protein.
Keyword
- NATURAL SCIENCES
- NATURVETENSKAP
Publication and Content Type
- ref (subject category)
- art (subject category)
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