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Real-time visualizi...
Real-time visualizing the regulation of reactive oxygen species on Zn2+ release in cellular lysosome by a specific fluorescent probe
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- Hu, Zhang-Jun (author)
- Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten
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- Yang, Guanqing (author)
- Anhui Univ, Peoples R China
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- Hu, Jiwen (author)
- Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten
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- Wang, Hui (author)
- Anhui Univ, Peoples R China
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- Eriksson, Peter (author)
- Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten
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- Zhang, Ruilong (author)
- Anhui Univ, Peoples R China
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- Zhang, Zhongping (author)
- Anhui Univ, Peoples R China
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- Uvdal, Kajsa (author)
- Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten
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(creator_code:org_t)
- ELSEVIER SCIENCE SA, 2018
- 2018
- English.
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In: Sensors and actuators. B, Chemical. - : ELSEVIER SCIENCE SA. - 0925-4005 .- 1873-3077. ; 264, s. 419-425
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https://doi.org/10.1...
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Abstract
Subject headings
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- Reactive oxygen species (ROS) regulating the release of free zinc ions (Zn2+) in cellular lysosome is closely related to various pathways of cellular signal transduction, such as inflammation and oxidative stress. Directly visualizing Zn2+ release in lysosome is essential for in-depth understanding these physiological processes, and is still an atelic challenge. In this work, we successfully fabricate a lysosome-specific Zn2+ fluorescent probe and achieve the visualization of ROS-induced Zn2+ release in lysosome of inflammatory cells. The as-prepared probe combines a green fluorophore, an ionophore with five-dentate sites, and a morpholine as the lysosome-specific localization moiety. The fluorescence of the fluorophore in the free probe is suppressed by a photoinduced electron transfer (PET) process from nitrogen atoms in the ionophore. Upon the addition of Zn2+, the fluorescence can be promoted immediately, achieving the real-time detection. Meanwhile, the probe is sensitive and selective to Zn2+, which provides the capability to detect low-concentration of free Zn2+ in lysosomes. Accordingly, the Zn2+ release was clearly observed in lysosome with the increase of ROS levels when the inflammation occurred in living cells. (c) 2018 Published by Elsevier B.V.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Fluorescent probe; Realtime visualization; Lysosome-specific Zn2+ probe; ROS regulation; Exogenous and endogenous Zn2
Publication and Content Type
- ref (subject category)
- art (subject category)
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