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  • Peris-Frau, PatriciaSaBio IREC CSIC UCLM JCCM, Spain (author)

Comparative evaluation of DNA integrity using sperm chromatin structure assay and Sperm-Ovis-Halomax during in vitro capacitation of cryopreserved ram spermatozoa

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • 2019-10-17
  • WILEY,2019
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:liu-161618
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-161618URI
  • https://doi.org/10.1111/rda.13519DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Funding Agencies|ALF-Research, Region Ostergotland [LIO-698951]; Lions Forskningsfond [DNR LIU-2016-00641]; Linkoping and the Swedish Research Council FormasSwedish Research Council Formas [2017-00946]; Forskningsradet i Sydostra Sverige [473121, 745971]; Universidad de Castilla-La Mancha [2015/4062]
  • This study aimed to compare the ability of sperm chromatin structure assay (SCSA (R)) and Sperm-Ovis-Halomax (R) to detect DNA fragmentation in frozen-thawed ram spermatozoa incubated under capacitating conditions in synthetic oviductal fluid (SOF) supplemented with oestrous sheep serum (SOF-ESS) at multiple time points (0-240 min). Incubation in SOF-ESS had no significant effects on SCSA (R) parameters while the percentage of spermatozoa with fragmented DNA measured by Sperm-Ovis-Halomax (R) increased after 180 min of incubation. In addition, no correlation or agreement was found between the techniques, suggesting that SCSA (R) and Sperm-Ovis-Halomax (R) may quantify different types of DNA damage in ram spermatozoa under these experimental conditions.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Alvarez-Rodriguez, ManuelLinköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten(Swepub:liu)manal80 (author)
  • Martin-Maestro, AliciaSaBio IREC CSIC UCLM JCCM, Spain (author)
  • Iniesta-Cuerda, MariaSaBio IREC CSIC UCLM JCCM, Spain (author)
  • Sanchez-Ajofrin, IreneSaBio IREC CSIC UCLM JCCM, Spain (author)
  • Julian Garde, JoseSaBio IREC CSIC UCLM JCCM, Spain (author)
  • Rodriguez-Martinez, HeribertoLinköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten(Swepub:liu)herro55 (author)
  • Josefa Soler, AnaSaBio IREC CSIC UCLM JCCM, Spain (author)
  • SaBio IREC CSIC UCLM JCCM, SpainAvdelningen för barns och kvinnors hälsa (creator_code:org_t)

Related titles

  • In:Reproduction in domestic animals: WILEY54, s. 46-490936-67681439-0531

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