Distinct Roles for Two Histamine Receptors (hclA and hclB) at the Drosophila Photoreceptor Synapse

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Pantazis, Antonios,1982-Department of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge CB2 3DY, United Kingdom (author)

Distinct Roles for Two Histamine Receptors (hclA and hclB) at the Drosophila Photoreceptor Synapse

Article/chapterEnglish2008

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Society for Neuroscience,2008
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LIBRIS-ID:oai:DiVA.org:liu-162197
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-162197URI
https://doi.org/10.1523/JNEUROSCI.1654-08.2008DOI

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Histamine (HA) is the photoreceptor neurotransmitter in arthropods, directly gating chloride channels on large monopolar cells (LMCs), postsynaptic to photoreceptors in the lamina. Two histamine-gated channel genes that could contribute to this channel in Drosophila are hclA (also known as ort) and hclB (also known as hisCl1), both encoding novel members of the Cys-loop receptor superfamily. Drosophila S2 cells transfected with these genes expressed both homomeric and heteromeric histamine-gated chloride channels. The electrophysiological properties of these channels were compared with those from isolated Drosophila LMCs. HCLA homomers had nearly identical HA sensitivity to the native receptors (EC50 = 25 ÎŒm). Single-channel analysis revealed further close similarity in terms of single-channel kinetics and subconductance states (~25, 40, and 60 pS, the latter strongly voltage dependent). In contrast, HCLB homomers and heteromeric receptors were more sensitive to HA (EC50 = 14 and 1.2 ÎŒm, respectively), with much smaller single-channel conductances (~4 pS). Null mutations of hclA (ortUS6096) abolished the synaptic transients in the electroretinograms (ERGs). Surprisingly, the ERG “on” transients in hclB mutants transients were approximately twofold enhanced, whereas intracellular recordings from their LMCs revealed altered responses with slower kinetics. However, HCLB expression within the lamina, assessed by both a GFP (green fluorescent protein) reporter gene strategy and mRNA tagging, was exclusively localized to the glia cells, whereas HCLA expression was confirmed in the LMCs. Our results suggest that the native receptor at the LMC synapse is an HCLA homomer, whereas HCLB signaling via the lamina glia plays a previously unrecognized role in shaping the LMC postsynaptic response.

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Segaran, AshvinaDepartment of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge CB2 3DY, United Kingdom (author)
Liu, Che-HsiungDepartment of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge CB2 3DY, United Kingdom (author)
Nikolaev, AntonDepartment of Biomedical Science, University of Sheffield, Sheffield S10 2TN, United Kingdom (author)
Rister, JensLehrstuhl für Genetik und Neurobiologie, Universität Würzburg, 97074 Würzburg, Germany (author)
Thum, Andreas S.Lehrstuhl für Genetik und Neurobiologie, Universität Würzburg, 97074 Würzburg, Germany (author)
Roeder, ThomasZoologisches Institut, Abteilung Zoophysiologie, Christian-Albrechts-Universität, D-24098 Kiel, Germany (author)
Semenov, EugeneDepartment of Molecular Neurobiology, Drosophila Neurogenetics Laboratory, Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria (author)
Juusola, MikkoDepartment of Biomedical Science, University of Sheffield, Sheffield S10 2TN, United Kingdom (author)
Hardie, Roger C.Department of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge CB2 3DY, United Kingdom (author)
Department of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge CB2 3DY, United KingdomDepartment of Biomedical Science, University of Sheffield, Sheffield S10 2TN, United Kingdom (creator_code:org_t)

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In:Journal of Neuroscience: Society for Neuroscience28:29, s. 7250-72590270-64741529-2401

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By the author/editor: Pantazis, Antoni ...; Segaran, Ashvina; Liu, Che-Hsiung; Nikolaev, Anton; Rister, Jens; Thum, Andreas S.; show more...; Roeder, Thomas; Semenov, Eugene; Juusola, Mikko; Hardie, Roger C.; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Neurosciences

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By the university: Linköping University

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