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Quantitative membra...
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Eriksson, HannaKarolinska Institutet
(author)
Quantitative membrane proteomics applying narrow range peptide isoelectric focusing for studies of small cell lung cancer resistance mechanisms
- Article/chapterEnglish2008
Publisher, publication year, extent ...
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Wiley,2008
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:liu-16263
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-16263URI
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https://doi.org/10.1002/pmic.200800174DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:117502778URI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:117982920URI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Drug resistance is often associated with upregulation of membrane-associated drug-efflux systems, and thus global membrane proteomics methods are valuable tools in the search for novel components of drug resistance phenotypes. Herein we have compared the microsomal proteome from the lung cancer cell line H69 and its isogenic Doxorubicin-resistant subcell line H69AR. The method used includes microsome preparation, iTRAQ labeling followed by narrow range peptide IEF in an immobilized pH-gradient (IPG-IEF) and LC-MS/MS analysis. We demonstrate that the microsomal preparation and iTRAQ labeling is reproducible regarding protein content and composition. The rationale using narrow range peptide IPG-IEF separation is demonstrated by its ability to: (i) lowering the complexity of the sample by two-thirds while keeping high proteome coverage (96%), (ii) providing high separation efficiency, and (iii) allowing for peptide validation and possibly identifications of post-transcriptional modifications. After analyzing one-fifth of the IEF fractions (effective pH range of 4.0-4.5), a total of 3704 proteins were identified, among which 527 were predicted to be membrane proteins. One of the proteins found to be differentially expressed was Serca 2, a calcium pump located in the ER membrane that potentially could result in changes of apoptotic response toward Doxorubicin.
Subject headings and genre
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Drug resistance
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Isoelectric focusing
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Lung cancer
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Membrane proteins
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Quantitative proteomics
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MEDICINE
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MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
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Lengqvist, JohanKarolinska Institutet
(author)
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Hedlund, JoelLinköpings universitet,Tekniska högskolan,Bioinformatik(Swepub:liu)joehe05
(author)
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Uhlen, KristinaGE Healthcare Biosci AB
(author)
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Orre, Lukas MKarolinska Institutet
(author)
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Bjellqvist, BengtKarolinska Institutet
(author)
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Persson, BengtKarolinska University Hospital
(author)
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Lehtio, JanneKarolinska Institutet
(author)
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Jakobsson, Per-JohanKarolinska Institutet
(author)
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Karolinska InstitutetTekniska högskolan
(creator_code:org_t)
Related titles
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In:Proteomics: Wiley28:5C, s. 3275-32761615-98531615-9861
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In:ANTICANCER RESEARCH: Wiley28:5C, s. 3275-32760250-7005
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