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Kinetic Screening o...
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Balian, AlienLinköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten,Wallenberg Centre for Molecular Medicine (WCMM), Sweden,Nucleic Acids Technologies Laboratory (NAT-lab)
(author)
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
- Article/chapterEnglish2019
Publisher, publication year, extent ...
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2019-11-01
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United States :JOURNAL OF VISUALIZED EXPERIMENTS,2019
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Numbers
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LIBRIS-ID:oai:DiVA.org:liu-162891
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-162891URI
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https://doi.org/10.3791/60005DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Funding Agencies|Knut and Alice Wallenberg FoundationKnut & Alice Wallenberg Foundation; Swedish Government Strategic Research Area in Materials Science on Advanced Functional Materials at Linkoping University [2009-00971]
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Nucleases are a class of enzymes that break down nucleic acids by catalyzing the hydrolysis of the phosphodiester bonds that link the ribose sugars. Nucleases display a variety of vital physiological roles in prokaryotic and eukaryotic organisms, ranging from maintaining genome stability to providing protection against pathogens. Altered nuclease activity has been associated with several pathological conditions including bacterial infections and cancer. To this end, nuclease activity has shown great potential to be exploited as a specific biomarker. However, a robust and reproducible screening method based on this activity remains highly desirable. Herein, we introduce a method that enables screening for nuclease activity using nucleic acid probes as substrates, with the scope of differentiating between pathological and healthy conditions. This method offers the possibility of designing new probe libraries, with increasing specificity, in an iterative manner. Thus, multiple rounds of screening are necessary to refine the probes design with enhanced features, taking advantage of the availability of chemically modified nucleic acids. The considerable potential of the proposed technology lies in its flexibility, high reproducibility, and versatility for the screening of nuclease activity associated with disease conditions. It is expected that this technology will allow the development of promising diagnostic tools with a great potential in the clinic.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Garcia Gonzalez, JavierLinköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten,Wallenberg Centre for Molecular Medicine (WCMM), Sweden,Nucleic Acids Technologies Laboratory (NAT-lab)(Swepub:liu)javga96
(author)
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Bastida, NoraLinköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten,Nucleic Acids Technologies Laboratory (NAT-lab)(Swepub:liu)norba54
(author)
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Akhtar, Khadija-Tul KubraLinköpings universitet,Institutionen för fysik, kemi och biologi,Tekniska fakulteten,Nucleic Acids Technologies Laboratory (NAT-lab)(Swepub:liu)n/a
(author)
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Borsa, Baris AtaLinköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten,Wallenberg Centre for Molecular Medicine (WCMM), Sweden,Nucleic Acids Technologies Laboratory (NAT-lab)(Swepub:liu)barbo70
(author)
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Hernandez, FrankLinköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten,Wallenberg Centre for Molecular Medicine (WCMM), Sweden,Nucleic Acids Technologies Laboratory (NAT-lab)(Swepub:liu)frahe49
(author)
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Linköpings universitetMolekylär ytfysik och nanovetenskap
(creator_code:org_t)
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In:Journal of Visualized ExperimentsUnited States : JOURNAL OF VISUALIZED EXPERIMENTS:1531940-087X
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