Light-Up Lipid Droplets Dynamic Behaviors Using a Red-Emitting Fluorogenic Probe

Zhang, Xin (author): Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten

Jiang, Jianxia (author): Linköpings universitet,Tunnfilmsfysik,Tekniska fakulteten,South China Univ Technol, Peoples R China

Hu, Jiwen (author): Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten

Du Rietz, Anna (author): Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten

Zhang, Fengling (author): Linköpings universitet,Biomolekylär och Organisk Elektronik,Tekniska fakulteten

Uvdal, Kajsa (author): Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten

Hu, Zhang-Jun (author): Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten

(creator_code:org_t)

2020-02-10
2020
English.
In: Analytical Chemistry. - : AMER CHEMICAL SOC. - 0003-2700 .- 1520-6882. ; 92:5, s. 3613-3619

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Abstract Subject headings

Intracellular lipid metabolism occurs in lipid droplets (LDs), which is critical to the survival of cells. Imaging LDs is an intuitive way to understand their physiology in live cells. However, this is limited by the availability of specific probes that can properly visualize LDs in vivo. Here, an LDs-specific red-emitting probe is proposed to address this need, which is not merely with an ultrahigh signal-to-noise (S/N) ratio and a large Stokes shift (up to 214 nm) but also with superior resistance to photobleaching. The probe has been successfully applied to real-time tracking of intracellular LDs behaviors, including fusion, migration, and lipophagy processes. We deem that the proposed probe here offers a new possibility for deeper understanding of LDs-associated behaviors, elucidation of their roles and mechanisms in cellular metabolism, and determination of the transition between adaptive lipid storage and lipotoxicity as well.

By the author/editor: Zhang, Xin; Yuan, Lin; Jiang, Jianxia; Hu, Jiwen; Du Rietz, Anna; Cao, Hongzhi; show more...; Zhang, Ruilong; Tian, Xiaohe; Zhang, Fengling; Ma, Yuguang; Zhang, Zhongping; Uvdal, Kajsa; Hu, Zhang-Jun; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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