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Combining endocanna...
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Larsson, Johan,1990-Linköpings universitet,Avdelningen för neurobiologi,Medicinska fakulteten
(author)
Combining endocannabinoids with retigabine for enhanced M-channel effect and improved KV7 subtype selectivity
- Article/chapterEnglish2020
Publisher, publication year, extent ...
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2020-05-04
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ROCKEFELLER UNIV PRESS,2020
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:liu-165683
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-165683URI
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https://doi.org/10.1085/jgp.202012576DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Funding agencies: Swedish Society for Medical Research; Swedish Research CouncilSwedish Research Council [2017-02040]
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Retigabine is unique among anticonvulsant drugs by targeting the neuronal M-channel, which is composed of KV7.2/KV7.3 and contributes to the negative neuronal resting membrane potential. Unfortunately, retigabine causes adverse effects, which limits its clinical use. Adverse effects may be reduced by developing M-channel activators with improved KV7 subtype selectivity. The aim of this study was to evaluate the prospect of endocannabinoids as M-channel activators, either in isolation or combined with retigabine. Human KV7 channels were expressed in Xenopus laevis oocytes. The effect of extracellular application of compounds with different properties was studied using two-electrode voltage clamp electrophysiology. Site-directed mutagenesis was used to construct channels with mutated residues to aid in the mechanistic understanding of these effects. We find that arachidonoyl-L-serine (ARA-S), a weak endocannabinoid, potently activates the human M-channel expressed in Xenopus oocytes. Importantly, we show that ARA-S activates the M-channel via a different mechanism and displays a different KV7 subtype selectivity compared with retigabine. We demonstrate that coapplication of ARA-S and retigabine at low concentrations retains the effect on the M-channel while limiting effects on other KV7 subtypes. Our findings suggest that improved KV7 subtype selectivity of M-channel activators can be achieved through strategically combining compounds with different subtype selectivity.
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Karlsson, Urban,1967-Linköpings universitet,Avdelningen för neurobiologi,Medicinska fakulteten(Swepub:liu)urbka85
(author)
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Wu, Xiongyu,1972-Linköpings universitet,Kemi,Tekniska fakulteten(Swepub:liu)xiowu62
(author)
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Liin, Sara,1982-Linköpings universitet,Medicinska fakulteten,Avdelningen för neurobiologi(Swepub:liu)sarbo42
(author)
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Linköpings universitetAvdelningen för neurobiologi
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In:The Journal of General Physiology: ROCKEFELLER UNIV PRESS152:80022-12951540-7748
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