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Seminal Plasma Modulates miRNA Expression by Sow Genital Tract Lining Explants

Barranco, Isabel (author)
Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,University of Murcia, Murcia, Spain; University of Girona, Girona, Spain
Padilla, Lorena (author)
Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,University of Murcia, Murcia, Spain
Martinez-Serrano, Cristina (author)
Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten
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Alvarez-Rodriguez, Manuel, 1983- (author)
Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten
Parrilla, Inmaculada (author)
University of Murcia, Murcia, Spain
Lucas, Xiomara (author)
University of Murcia, Murcia, Spain
Ferreira-Dias, Graça (author)
University of Lisbon, Lisbon, Portugal
Yeste, Marc (author)
University of Girona, Girona, Spain
Rodriguez-Martinez, Heriberto, 1950- (author)
Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten
Roca, Jordi (author)
University of Murcia, Murcia, Spain
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 (creator_code:org_t)
2020-06-19
2020
English.
In: Biomolecules. - : MDPI. - 2218-273X. ; 10:6
Related links:
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • The seminal plasma (SP) modulates the female reproductive immune environment after mating, and microRNAs (miRNAs) could participate in the process. Considering that the boar ejaculate is built by fractions differing in SP-composition, this study evaluated whether exposure of mucosal explants of the sow internal genital tract (uterus, utero-tubal junction and isthmus) to different SP-fractions changed the profile of explant-secreted miRNAs. Mucosal explants retrieved from oestrus sows (n = 3) were in vitro exposed to: Medium 199 (M199, Control) or M199 supplemented (1:40 v/v) with SP from the sperm-rich fraction (SRF), the post-SRF or the entire recomposed ejaculate, for 16 h. After, the explants were cultured in M199 for 24 h to finally collect the media for miRNA analyses using GeneChip miRNA 4.0 Array (Affymetrix). Fifteen differentially expressed (False Discovery Rate (FDR) < 0.05 and Fold-change ≥ 2) miRNAs (11 down- versus 4 up-regulated) were identified (the most in the media of uterine explants incubated with SP from post-SRF). Bioinformatics analysis identified that predicted target genes of dysregulated miRNAs, mainly miR-34b, miR-205, miR-4776-3p and miR-574-5p, were involved in functions and pathways related to immune response. In conclusion, SP is able to elicit changes in the miRNAs profile secreted by female genital tract, ultimately depending SP-composition.

Subject headings

LANTBRUKSVETENSKAPER  -- Veterinärmedicin (hsv//swe)
AGRICULTURAL SCIENCES  -- Veterinary Science (hsv//eng)

Keyword

explants
female genital tract
immune response
miRNAs
mucosal tissue
seminal plasma
transcriptome

Publication and Content Type

ref (subject category)
art (subject category)

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