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- Padilla, LorenaUniv Murcia, Spain (author)
Granulocyte-macrophage colony stimulating factor (GM-CSF) is fully expressed in the genital tract, seminal plasma and spermatozoa of male pigs
- Article/chapterEnglish2020
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- 2020-08-07
- NATURE PUBLISHING GROUP,2020
- electronicrdacarrier
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- LIBRIS-ID:oai:DiVA.org:liu-169235
- https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-169235URI
- https://doi.org/10.1038/s41598-020-70302-9DOI
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- Language:English
- Summary in:English
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- Funding Agencies|MINECO Madrid (Spain) [AGL2015-69738-R]; FEDER Madrid (Spain) [AGL2015-69738-R]; Seneca Foundation Murcia (Spain)Fundacion Seneca [19892/GERM/15]; Swedish Research Council FORMAS, Stockholm, Sweden [2017-00946, 2019-00288]; MINECO (Madrid, Spain)
- Granulocyte-macrophage colony stimulating factor (GM-CSF) is a pro-inflammatory cytokine identified in boar seminal plasma (SP) but until now unexplored in terms of place of production and its association to spermatozoa. This study aimed to explore these aspects by evaluating the presence of GM-CSF in porcine reproductive organs (testes, epididymis and accessory sex glands), SP and mature spermatozoa (from cauda epididymis and ejaculated) using Western blot (WB), immunohistochemistry and immunocytochemistry. Positive labelling was obtained in tissues, SP and spermatozoa. In reproductive organs, WB revealed three forms of GM-CSF with different glycosylation degrees (15, 31 and 40 kDa). In SP and epididymal fluid, the GM-CSF appeared only in its active form while in spermatozoa the GM-CSF form present varied among sperm sources. Non-viable spermatozoa showed more GM-CSF than viable spermatozoa (14.87 +/- 1.98 RU vs. 7.25 +/- 0.52 RU) of fluorescence intensity. In conclusion, GM-CSF is widely present in the reproductive tract of male pigs, attached to the spermatozoa already in the epididymis as well as verted to SP. Consequently, the GM-CSF ought to regulate male genital tract and sperm function as well as mediating initial inflammatory responses and further mediating later immune actions by the female to semen deposition.
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- Martinez-Hernandez, JesusUniv Murcia, Spain (author)
- Barranco, IsabelUniv Murcia, Spain; Univ Girona, Spain (author)
- Lucas, XiomaraUniv Murcia, Spain (author)
- Pastor, Luis M.Univ Murcia, Spain (author)
- Rodriguez-Martinez, HeribertoLinköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten(Swepub:liu)herro55 (author)
- Roca, JordiUniv Murcia, Spain (author)
- Parrilla, InmaculadaUniv Murcia, Spain (author)
- Univ Murcia, SpainUniv Murcia, Spain; Univ Girona, Spain (creator_code:org_t)
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- In:Scientific Reports: NATURE PUBLISHING GROUP10:12045-2322
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